EN 13697-Bactericidal and/or fungicidal activity on non-porous surfaces. UNE-EN 13697:2015. Chemical disinfectants and antiseptics. Quantitative non-porous surface test for the evaluation of bactericidal and/or fungicidal activity of chemical disinfectants used in food, industrial, domestic and institutional areas. Test method and requirements without mechanical action (phase 2, step 2).
Test accredited by ENAC (National Accreditation Entity).
The test organisms used as mandatory to determine the bactericidal activity are four bacteria (Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus and Enterococcus hirae) and to determine the fungicidal activity two fungi (Candida albicans and Aspergillus brasiliensis). The yeasticidal activity can also be determined using only Candida albicans. Optional additional bacteia and fungi are: Salmonella enterica serov. typhimurium and Enterobacter cloacae Lactobacillus brevis, Saccharomyces cerevisiae (breweries) Saccharomyces cerevisiae var. diastaticus (breweries). The test should be performed at a temperature between 18ºC and 25ºC and a time of 5 minutes for bactericidal activity or 15 minutes for fungicidal activity. For bactericidal and/or fungicidal activity optional additional temperatures of 4, 10 or 40ºC and optional additional times of 1, 5, 15, 30 and 60 minutes can be used. The test can be performed under clean or dirty conditions. The product should be tested at least at three concentrations including one in the non-active interval and another in the active interval. The product is tested on stainless steel discs and bacterial or fungal viability is evaluated by the method of dilution-neutralization. To consider the product bactericidal must demonstrate a log reduction of 4 in the number of viable bacteria and to consider the product fungicidal must demonstrate a 3 log reduction in the number of viable cells or fungal conidia.