EN 1276-Bactericidal activity under use conditions. UNE-EN 1276: 2010 ERRATUM: 2011. Chemical disinfectants and antiseptics. Quantitative suspension test for the evaluation of bactericidal activity of chemical disinfectants and antiseptics used in food, industrial, domestic and institutional areas. Test method and requirements (phase 2, step 1).

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Chemical disinfectants and antiseptics. Quantitative suspension test for the evaluation of bactericidal activity of chemical antiseptics and disinfectants used in food, industry, at home and in the community. Test method and requirements - Phase 2, Step 1). The test organisms used as required Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus and Enterococcus hirae, and as optional additional Salmonella typhimurium, Lactobacillus brevis and Enterobacter cloacae. The test should be performed at a temperature of 20 ° C and mandatory for a mandatory period of 5 minutes, except for hand disinfection which is 1 minute. It can also be done with optional additional temperatures of 4, 10 or 40 and optional additional times for 1, 15, 30 or 60 minutes. The test can be done in a clean condition or dirty conditions. The product should be tested at least three levels including the non - active interval and another in the active interval. The product can be assayed by the method of dilution-neutralization method or by membrane filtration. To consider the bactericidal product must demonstrate a log reduction of 5 in the number of viable cells.