Instituto Valenciano de Microbiología

Masía El Romeral
Ctra. de Bétera a San Antonio Km. 0.3
46117 Bétera (Valencia)
Phone. 96 169 17 02
Fax 96 169 16 37
CIF B-96337217


Bactericidal activity under use conditions. NF EN 1276: 2019 - Chemical disinfectants and antiseptics. Quantitative suspension test for the evaluation of bactericidal activity of chemical disinfectants and antiseptics used in food, industrial, domestic and institutional areas.      Test method and requirements (phase 2, step 1).

Test accredited by ENAC (Spanish National Accreditation Entity).

For general disinfection products, this test uses as mandatory organisms: Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus and Enterococcus hirae, or only Enterococcus faecium for temperatures ≥ 40ºC. Salmonella typhimurium, Lactobacillus brevis and Enterobacter cloacae, may be included as additional microorganisms. The test should be performed at a temperature within the range from 4°C to 60°C and for a time of 1 to 60 minutes (from 1 to 5 minutes at 1minute intervals and from 5 to 60 minutes at intervals of 5 minutes). The test can be performed in clean or dirty conditions. For hand hygiene products, the test uses Pseudomonas aeruginosa, Escherichia coli K12, Staphylococcus aureus and Enterococcus hirae as mandatory microorganisms. For this application, the test is performed at a temperature of 20ºC, the contact time should be 30 or 60 seconds, and the mandatory soiling conditions are clean conditions for hygienic handrub products and dirty conditions for hygienic handwashing products. The product must be tested at least at three concentrations including one in the non-active range and another in the active range (these two concentrations are chosen by the laboratory that performs the test as they are the positive and negative controls of the test, respectively). The product can be tested by the dilution-neutralization method, or by the membrane filtration method. General disinfection products and handrub products must demonstrate at least a 5 log reduction in the number of viable cells to be considered as bactericidal, whereas handwashing products must demonstrate at least a 3 log reduction. Products can be tested at a concentration equal or lower than 80%. The maximum test concentration of products for hygienic handwashing is 50%.

Tests with guideline mandatory bacteria

Tests with non-mandatory bacteria