Instituto Valenciano de Microbiología

Masía El Romeral
Ctra. de Bétera a San Antonio Km. 0.3
46117 Bétera (Valencia)
Phone. 96 169 17 02
Fax 96 169 16 37
CIF B-96337217


ASTM E2274-16. Standard test Method for evaluation of laundry sanitizers and disinfectants.

Test accredited by ENAC (National Accreditation Entity).

               This method is designed to evaluate the disinfectants or sanitary products of laundries used in automatic washing processes using volumes of usual wash water, applied to demonstrate the reduction or complete death of a bacterial population in tissues and wash water after a single wash. This method is designed for tests with vegetative bacteria but can be adapted for tests with fungi or viruses. The method simulates the washing conditions, for which a set of tissues inoculated with the microorganisms is placed in a volume of the solution of the evaluated product, in the proportion indicated by the manufacturer, and shaken. After a period of contact time, the washing water, the neutralizing liquid, and the tissue used, are individually grown in a quantitative manner (cleaning efficiency), or qualitatively (disinfecting efficacy). The following bacterial species are used: Klebsiella pneumoniae, Staphylococcus aureus, and Pseudomonas aeruginosa, although other bacteria of choice may be included. The test can be carried out in clean conditions (without organic matter), or in dirty conditions (using some organic matter among the indicated alternatives: defibrinated heat-inactivated bovine serum or a mixture of tryptone, bovine serum albumin and mucin may be used). Six pieces of tissue are used for each microorganism. Three pieces for the test product and 3 pieces for the controls. These inoculated pieces are inserted between the folds of a 15 g piece of cloth and maintained inside the closed exposure chamber under agitation for the time specified by the manufacturer. Once the exposure time has elapsed, the supports and the inoculated pieces of tissue are removed and introduced into a neutralization broth. The filtrate of the neutralization broth, the neutralization broth itself and dilutions prepared therefrom, are cultivated to detect the presence of the inoculated microorganisms, as well as their reduction, if any, expressed in log10. Duplicate validations of the microorganism count controls are included in the test. The counting of the surviving microorganisms after the washing simulation process is carried out by means of quantitative counts in duplicate, with 5 dilutions in base 10.

Cost of tests with the microorganism indicated by the ASTM guideline:

Cost of other options not required by the guideline (not individually accredited by ENAC*)