ASTM E2406-Evaluation of laundry sanitizers and disinfectants for high efficiency washingASTM E2406-16Standard test method for evaluation of laundry sanitizers and disinfectants for use in high efficiency washing operations.

Test accredited by ENAC (National Accreditation Entity).

This method is designed to evaluate the sanitizers and disinfectants for laundries used in automatic processes washes for clothes with high efficiency using very small volumes of washings water, for demonstrating the reduction or complete death of a bacterial population in tissues and washing water after a single wash. This method is designed for testing vegetative bacteria, but can be adapted for testing fungi or viruses. The method simulates the washing conditions to which a set of tissues inoculated with microorganisms are placed in a small volume of the product solution under evaluation in the proportions indicated by the manufacturer and stirred. After a period of contact time, the washing water, the neutralization solution, and the fabric used, are individually cultured quantitatively (cleaning effectiveness), or qualitatively (disinfecting efficacy). The following species of bacteria are used: Klebsiella pneumoniae, Staphylococcus aureus, and Pseudomonas aeruginosa, but can include other bacteria choice. The test can be performed under clean conditions (without organic matter), or dirty conditions (using any organic matter from the indicated alternatives). Six fabric pieces are used for each microorganism; three pieces for the test product; and three pieces for the controls. These inoculated pieces are inserted between the folds of a fabric piece of 15 g. The chamber of exposure closed is stirred during the time specified by the manufacturer. After expiry of the exposure time, the inoculated tissue pieces are removed and placed in a neutralizing broth. The neutralized broth filtrate, the neutralization broth itself and dilutions prepared from them, are cultured for the presence of the inoculated microorganisms, and their reduction if expressed in log10. In the test validations, duplicate controls include the microbial count. The counting of surviving microorganisms after the washing process simulation is performed by quantitative counts in duplicate with 5 base 10 dilutions.