EN 16437-Bactericidal activity in veterinary area on porous surfaces. UNE-EN 16437: 2014 Chemical disinfectants and antiseptics. Quantitative surface test for the evaluation of bactericidal activity of chemical disinfectants and antiseptics used in veterinary area on porous surfaces without mechanical action. Test method and requirements (phase 2, step 2).

Test accredited by ENAC (National Accreditation Entity).

This standard establishes the procedure to know if an antiseptic or disinfectant used in the veterinary area on porous surfaces without mechanical action, has bactericidal activity given the practical conditions of use, which can influence its action such as drying of microorganisms on the surface, the presence of interfering substances, the contact time and temperature. The products tested under these conditions are those that can be used in veterinary applications on porous surfaces without the use of mechanical actions, in situations such as rearing, exploration, production, transportation and disposal of animals, except when in the chain food after slaughter and entry into the processed food industry. To be tested, the products should form a physically stable homogeneous preparation when diluted in hard water, or water in the case of products ready for use. The method uses as mandatory test microorganisms Enterococcus hirae, Staphylococcus aureus, Proteus vulgaris and Pseudomonas aeruginosa, but other optional desired microorganisms may be included. The assay temperature must be 10ºC, but can also be tested at 4, 20 or 40ºC. The contact time required is 60 minutes, but may be included as additional 1, 5, 15, 30 or 120 minutes. An interfering substance must be included representing dirty conditions indicated by the standard, and any additional desired. To considered the product active in the test conditions specified by this standard, a reduction of 4 log10 in the number of microorganisms, for the four indicated species of bacteria must be obtained, but according to choice a 3 log10 may be enough.