Virucidal activity in the veterinary area against Newcastle disease virus according to UNE-EN 14675: 2015 methods - Antiseptics and chemical disinfectants. Quantitative suspension test for the evaluation of the virucidal activity of the antiseptics and chemical disinfectants used in the veterinary area. Test method and requirements -phase 2, step 1-).
Test not accredited in our laboratory (see note 1 for accredited test).
This UNE-EN test uses bovine Enterovirus type 1 as an obligate virus. Additional optional viruses proposed by the client may be used, as in this case, virucidal assay with Newcastle disease virus disease of viral origin that affects birds and occasionally It can be transmitted to people.
The test must be carried out at a mandatory temperature of 10ºC and for a mandatory time of 30 minutes. Optional additional temperatures may be chosen as 4°C, 20°C or 40°C, and as optional additional times 1, 5 or 60 minutes may be used. The test can be performed under simulated conditions of low dirt or in simulated conditions of high dirt. The product should be tested at least three concentrations including one in the active range and one in the non-active range (these last two concentrations are chosen by the laboratory that performs the test since they correspond to the positive and negative controls, respectively). To consider the virucidal product a logarithmic reduction of 4 in infectivity must be demonstrated.
Note 1: For those optional virus not mandatory by the standard guideline, to be under accreditation, it is required that the test be performed at the same time with any of the mandatory viruses.