Virucidal activity against Avian Influenza A H1N1 virus in the veterinary area (Standard UNE-EN 14675: 2015 -Antiseptics and chemical disinfectants. Quantitative suspension test for the evaluation of the virucidal activity of the antiseptics and chemical disinfectants used in the veterinary area. Test method and requirements -phase 2, step 1-).
Test not accredited in our laboratory (see Note 1* for accredited test).
The UNE-EN 14675 test uses bovine Enterovirus type 1 as an obligate organism. Additional optional viruses proposed by the client may be used, as in this case, virucidal test with Avian Influenza A H1N1 virus, disease caused by the virus infection of the virus. Influenza A that causes bird flu. This virus is found naturally among waterfowl around the world and can infect domestic poultry and other birds or other animal species.
The test must be carried out at a mandatory temperature of 10ºC and for a mandatory time of 30 minutes. Optional additional temperatures may be chosen as 4°C, 20°C or 40°C, and as optional additional times 1, 5 or 60 minutes may be used. The test can be performed under simulated conditions of low dirtiness or in simulated conditions of high dirtiness. The product should be tested at least three concentrations including one in the active range and one in the non-active range (these last two concentrations are chosen by the laboratory that performs the test since they correspond to the positive and negative controls, respectively). To consider the virucidal product a logarithmic reduction of 4 in infectivity must be demonstrated.
Note 1*: For those optional virus not mandatory by the standard guideline, to be under accreditation, it is required that the test be performed at the same time with any of the mandatory viruses.