Virucidal activity against African Swine fever virus using the Vaccinia virus as the surrogate virus, in the Veterinary area (UNE-EN 14675: 2015 guideline) 

Test not accredited by ENAC (National Accreditation Entitity) (see Note 1* below for testing being accredited).

The UNE-EN 14675 test uses bovine Enterovirus type 1 as an obligate organism. Additional optional viruses proposed by the client can also be used, as in this case, a virucide assay with an African swine fever surrogate virus, such as the Vaccinia virus. The African swine fever virus cannot be used in the laboratory for this type of tests due to its high contagiousness, and it is not even available for distribution in virus collections.

The African Swine Fever virus belongs to a different family: Asfarviridae, genus Asfivirus, with only one species and several strains in the same species. It is a complex virus with a different structure and a DNA genome, more similar to Poxviruses, such as Vaccinia virus. This is the reason to recommend this surrogate virus for testing disinfectans addressed to be used against African swine fever virus.

The test must be carried out at a mandatory temperature of 10ºC and for a mandatory time of 30 minutes. Optional additional temperatures may be chosen as 4°C, 20°C or 40°C, and as optional additional times 1, 5 or 60 minutes may be used. The test can be performed under simulated conditions of low dirtiness or in simulated conditions of high dirtiness. The product should be tested at least three concentrations including one in the active range and one in the non-active range (these last two concentrations are chosen by the laboratory that performs the test since they correspond to the positive and negative controls, respectively). To consider the virucidal product a logarithmic reduction of 4 in infectivity must be demonstrated. 

Note 1*: For those optional viruses not mandatory by the standard guideline, to be under accreditation, it is required that the test be performed at the same time with any of the mandatory viruses.