EN 1275-Basic fungicidal activity. Chemical disinfectants and antiseptics. UNE-EN 1275: 2007. Quantitative suspension test for the evaluation of basic fungicidal or basic yeasticidal activity of chemical disinfectants and antiseptics. Test method and requirements (phase 1).
Test accredited by ENAC (National Accreditation Entity).
This test organisms used as required for fungicidal activity Candida albicans and Aspergillus niger, (syn. Aspergillis brasiliensis) and Candida albicans for yeasticidal activity. The test should be performed at a mandatory temperature of 20ºC and for a mandatory time of 15 minutes. It can also be done with optional additional temperatures of 4, 20 or 40ºC and optional additional times of 1, 5, 30 or 60 minutes. The product should be tested at least three concentrations including one in the non-active interval and one in the active interval other with a downward geometric progression factor of at least 2-fold dilution with each of the above. The product can be assayed by the method of filtration-neutralization, or by the membrane filtration method. To consider the basic fungicidal activity the product must demonstrate a log reduction of 4 in the number of viable cells.