Instituto Valenciano de Microbiología

Masía El Romeral
Ctra. de Bétera a San Antonio Km. 0.3
46117 Bétera (Valencia)
Phone. 96 169 17 02
Fax 96 169 16 37
CIF B-96337217


Surface disinfection by air (Standard Guideline NF T72 281: 2014- Surface disinfection procedures by air. Determination of bactericidal, fungicidal, mycobactericidal, tuberculocidal, sporicidal and virucidal including bacteriophages, activities. AFNOR).

NOTE: This test was cancelled by AFNOR (France) in April, 2020 and replaced by EN 17272.

The disinfectant activity of a disinfection process by air (bactericidal, yeasticidal or sporicidal), should reduce the number of microorganisms indicated in the conditions required by the standard, corresponding to the strains of bacterial species, fungi, mycobacteria and viruses including bacteriophages, on a smooth reference carrier. The manufacturer must indicate the exposure time of the test product. The microorganisms used are: bactericidal activity (Pseudomonas aeruginosa, Staphylococcus aureus, Enterococcus hirae, Escherichia coli); Sporicidal activity (Bacillus subtilis spores); Levuricidal activity (Candida albicans); Fungicidal activity (Candida albicans, Aspergillus brasiliensis), mycobactericidal and tuberculicidal activity (Mycobacterium avium and Mycobacterium terrae), virucidal (Bovine Enterovirus type 1, murine Norovirus, Adenovirus type 5), activity against bacteriophages (Lactobacillus lactis bacteriophages).

In case of testing products intended for the human health sector (products type 2, PT2, for the medical area) the standard establishes as mandatory the clean conditions (milk 1/20, except for viruses that should be tested with 0.3 g/L of BSA) and includes the dirty conditions as additional conditions (with 3 g/L of BSA plus 3 mg/L of erythrocytes). In case of testing products for the veterinary sector (products type 3, PT3), the AFNOR standard establishes as mandatory the conditions of low level of soiling (using as interfering substance 0.3 g/L of BSA) and the high-level dirty conditions as additional, (in the presence of 10 g/L of BSA plus 10 g/L of yeast extract). In case of testing products for the agri-food area, industry, and communities (products type 2, PT2 for the domestic and industrial area and products type 4, PT4 for food and feed) the French standard states as mandatory the study under clean conditions (milk 1/20, except for viruses that should be tested with 0.3 g/L of BSA), and stablish as additional conditions the test under dirty conditions (with 3 g/L of BSA). The emission devices required for each test are: 6 emission devices for the bactericidal activity and 4 emission devices for each of the other tests. In case of testing, the emission devices must be adequate for the volume of the chamber of assay used in the laboratory (74 m3; seventy-four cubic meters).    

If you want to perform the analysis according to the BPR Guidance (Guidance on the Biocidal Products Regulation), you must bear in mind that the recommended microorganisms may change depending on the type of product to be analyzed. Thus, for the analysis of the bactericidal activity, for the PT3 products, the BPR guidance recommends to perform the test using Proteus vulgaris, instead of Escherichia coli, while for the analysis of PT2 products for use in the medical area, the BPR guide recommends to perform the test with Escherichia coli K12, instead of with Escherichia coli. Furthermore, the BPR guide indicates modifications of other microorganisms with respect to the AFNOR Standard. The customer must consult with the authorities the microorganisms needed for each assay. In our laboratory we have the AFNOR NF T72 281: 2014 standard accredited by ENAC, except for activity against viruses and bacteriophages. Therefore, to perform the assay without deviations from the standard, at least the mandatory microorganisms, required by the AFNOR NF Standard T72 281: 2014, must be analyzed.