Quantitative bactericidal activity on porous surface in the veterinary area (UNE-EN 16437: 2014 + A1: 2020. Chemical disinfectants and antiseptics. Quantitative surface test for the evaluation of bactericidal activity of chemical disinfectants and antiseptics used in veterinary area on porous surfaces without mechanical action. Test method and requirements (phase 2, step 2).  

Test accredited by ENAC (National Accreditation Entity)

This standard establishes the procedure to know if an antiseptic or disinfectant used in the veterinary area on porous surfaces, without mechanical action, has bactericidal activity taking into account the practical conditions of use, that can influence its action as the drying of the microorganisms on the surface, the presence of interfering substances, the contact time and the temperature. The products tested under these conditions are those that can be used in veterinary applications on porous surfaces without the use of mechanical actions in situations such as breeding, exploitation, production, transport and disposal of animals, except when they are in the food chain after their slaughter and entry into the processed food industry. In order to be tested, the products must form a homogeneous preparation which is physically stable when diluted in hard water or in water in the case of ready-to-use products. The method uses Enterococcus hirae, Staphylococcus aureus, Proteus vulgaris and Pseudomonas aeruginosa as mandatory microorganisms, but other optional microorganisms may be included as desired. The mandatory test temperature is 10°C, although it may be also tested at 4°C, 20°C or 40°C. The minimum contact time is 1 minute and the maximum 360 minutes. In this range it can be chosen contact times of 5, 15, 30 and 60 minutes, and up to 360 minutes at 30 minutes in between. There must be an inherent substance of dirty conditions indicated by the standard, and any other additional desired. For the product to be considered active under the test conditions indicated by this standard, it must achieve a reduction of the number of microorganisms, for the four species of bacteria indicated, of 4 log10.