Virucidal activity against foot and mouth disease virus (Foot and Mouth Disease) with Enterovirus 71, in the veterinary area (Standard UNE-EN 14675: 2015 - Antiseptics and chemical disinfectants - Quantitative suspension test for the evaluation of virucidal activity of antiseptics and chemical disinfectants used in the veterinary area. Test method and requirements - phase 2, step 1-).

Test accredited by ENAC (National Accreditation Entity).

The UNE-EN 14675 test uses bovine Enterovirus type 1 as an obligate organism. Additional optional viruses proposed by the client may be used, as in this case, a virucide assay with Enterovirus 71 as a representative of one of the viruses that cause the disease Foot and Mouth (Foot and Mouth disease). The main viruses that cause this disease are different species and serotypes of the Picornaviridae family, being the most prevalent some serotypes of the Enterovirus A species, such as Coxsackievirus A 16 (CA16) and Enterovirus 71 (E71) and with a lower prevalence Coxsackievirus A5 (CA5), Coxsackievirus A10 (CA10) and Enterovirus B serotype Coxsackievirus B3. In our laboratory, it is used to perform the Enterovirus 71 tests.

The test must be carried out at a mandatory temperature of 10ºC and for a mandatory time of 30 minutes. Optional additional temperatures may be chosen as 4°C, 20°C or 40°C, and as optional additional times 1, 5 or 60 minutes may be used. The test can be performed under simulated conditions of low dirtiness or in simulated conditions of high dirtiness. The product should be tested at least three concentrations including one in the active range and one in the non-active range (these last two concentrations are chosen by the laboratory that performs the test since they correspond to the positive and negative controls, respectively). To consider the virucidal product a logarithmic reduction of 4 in infectivity must be demonstrated.