EN 14675-Virucidal activity against Classical Swine Fever virus (Hog chlolera) in the veterinary area using the surrogate BVDV (Bovine Viral Diarrhea virus) (UNE-EN 14675: 2007 – Chemical disinfectants and antiseptics. Quantitative suspension test for the evaluation of virucidal activity of chemical disinfectants and antiseptics used in the veterinary area. Test method and requirements, phase 2, step 1).

Test accredited by ENAC (National Accreditation Entity).

The UNE-EN 14675 test uses Bovine Enterovirus type 1 as an obligate virus. Additional optional viruses proposed by the client may be used, as in this case, the test with the African Classical Fever virus (SFV or Classical Swine Fever virus) (Hog chlolera). However, this virus cannot be used in the laboratory for this type of tests because of its high contagiousness, which is why it is not even available for distribution in virus collections.

Classical Swine Fever virus (Hog cholera) belongs to the family Flaviviridae, genus Pestivirus, the same family/genus as bovine viral diarrhea virus (BVDV). When a disinfectant test for SFV is required, it is possible to use BVDV as a surrogate virus and tested according to EN 14675 (virucidal test in the veterinary area). Our recommendation is to perform the virucidal activity test using the Bovine viral Diarrhea virus as a surrogate virus.

The test must be carried out at a mandatory temperature of 10ºC and for a mandatory time of 30 minutes. Optional additional temperatures may be chosen as 4°C, 20°C or 40°C, and as optional additional times 1, 5 or 60 minutes may be used. The test can be performed under simulated conditions of low dirt or in simulated conditions of high dirt. The product should be tested at least three concentrations including one in the active range and one in the non-active range (these last two concentrations are chosen by the laboratory that performs the test since they correspond to the positive and negative controls, respectively). To consider the virucidal product a logarithmic reduction of 4 in infectivity must be demonstrated.