Standard Practice for Determining Antimicrobial Efficacy of Ultraviolet Germicidal Irradiation Against Microorganisms on Carriers with simulated Soil (ASTM E3135–18).
Test not accredited yet in our laboratory.
ASTM E3135 defines the test conditions for evaluating germicidal ultraviolet light irradiation devices (mercury vapor bulbs, light emitting diodes, or xenon arc lamps), which are designed to kill or inactivate microorganisms deposited on inanimate supports.
This standard does not recommend either test microorganisms or soiling conditions, but rather indicates that the test microorganisms and soiling conditions, if any, should be selected by the customer based on the specific objectives of the UV irradiation device performance antimicrobial assay evaluation. As an example, two soiling agents are mentioned, a hydrophilic soiling agent, artificial saliva, and a waxy hydrophobic agent, artificial sebum, but indicates that appropriate soiling conditions should be chosen for the actual application of the irradiation device. The ASTM E3135 standard also indicates that the assay can be performed without using any interference substance. Depending of the application area of the equipment, the customer can orden the use o other interferences substance differents from those indicated by the EN or BPR guidelines (as example, bovine serum albumin 3 g/L; bovine serum albumin 0.3 g/L; bovine serum albumin 3 g/L + erythrocytes 3 mL/L; or bovine serum albumin 10 g/L + yeast extract 10 g/L).
The test described by ASTM E3135 is carried out on 2.5 to 3 cm diameter carriers of any material representative of the type of surface to which the UV irradiation device will be destined in its actual use. To perform the test, 6 carriers are used per test microorganism and soil condition (3 of them will be exposed to the UV light source, and the remaining three will serve as controls). The test microorganism is deposited on each carrier and, if applicable, once the inoculum has dried, the soiling agent. Three carriers of each selected condition (no soiling, hydrophilic soiling, hydrophobic soling or other soiling conditions indicated by the client) are exposed to ultraviolet light. Exposure should be conducted in accordance with the instructions on the label of the irradiation device and should simulate actual use of the device. The three control carriers for each condition are incubated at room temperature during the exposure time. At the end of the exposure period, the microorganisms are extracted from the supports and the number of surviving microorganisms is quantified, by cultivation in media appropriate for bacteria and fungi, or by titration in cell cultures appropriate for viruses. With these counts, the percentage reduction for the test microorganism due to UV treatment under a specific soil condition is calculated, by comparing the mean viable microorganisms recovered from the untreated control carriers, with the mean viable microorganisms recovered from the UV-treated carriers for a specific condition.
In our laboratory, these tests are carried out in a 74 m3 chamber. In general, at IVAMI these tests are performed on stainless steel carriers, if the client wishes to carry out the test on another type of carrier, to adapt it to the conditions of use of their device, they can consult us or provide us with the test carriers. This test is usually performed to test UV irradiation device efficacy against bacteria, yeast, fungi and viruses indicated below. If you need to perform the test with another microorganisms or viruses, consult us. The customer must indicate the irradiation procedure to follow with his product (irradiation time, distance from the UV source to the test supports, the relative angle of exposure to ultraviolet light, and the position of the supports relative to the length of the device) and the test conditions (test microorganisms, dirt conditions to be used, the material of which the test supports must be made, initial test temperature, and initial relative humidity range).
Cost of tests with bacteria, spores, fungi, or viruses mandatory by the standard guidelines or the BPR: 2018 guidance
- Basic price for bactericidal activity with four bacteria: consult.
- Basic price for bactericidal activity with three bacteri: consult.
- Basic price for bactericidal activity with two bacteria: consult.
- Basic price for bactericidal activity with one bacteria: consult.
- Basic price for yeasticidal activity (Candida albicans): consult.
- Basic price for fungicidal activity, with only Aspergillus brasiliensis: consult.
- Basic price for fungicidal activity (Candida albicans and Aspergillus brasiliensis): consult.
- Basic price for virucidal activity with three viruses indicated by the guideline: consult.
- Basic price for virucidal activity with only two viruses indicated by the guideline (any of them): consult.
- Basic price for virucidal activity with only one virus indicated by the EN guidelines (any of them): consult.
- Basic price for other viruses indicated by the BPR guidance for special uses (Poliovirus type 1, Murine Parvovirus, Rotavirus) (each one): consult.
Cost of tests with other bacteria, spores, fungi or viruses not mandatory by the EN standards or the BPR: 2018 guidance:
- Basic price with Staphylococcus aureus, Escherichia coli, Enterococcus faecalis y Bacillus subtilis (spores) (in total cuatro tests): consult.
- Basic price with one non-mandatory bacteria by any of the other EN standard for bactericidal activity (e. gr. Listeria monocytogenes; Bacillus cereus; Salmonella enterica subsp. enterica), each one: consult.
- Basic price with one fungus not mandatory by this standard, neither by any of the other EN standard for fungicidal activity (e. gr. Rhizopus
- Basic price with other two fungal species (e. gr. Penicillium italicum and Penicillium digitatum): consult.
- Basic price for yeasticidal activity with Saccharomyces cerevisia: consult.
- Basic price for sporicidal activity (Bacillus subtilis spores): consult.
- Basic price for mycobactericidal and tuberculicidal activity: consult.
- Basic price with Coronavirus humano 229E: consult.
- Basic price with Influenza A H1N1 virus: consult.
- Basic price with NewCastle Disease virus (Avulavirus): consult.
- Basic price with PRRSV (Porcine Reproductive Respiratory Syndrome virus): consult.
- Basic price with Vaccinia virus: consult.
- Basic price for activity against African Swine fever virus using the surrogate Vaccinia virus: consult.
- Basic price for activity against Infectious Bursal Disease virus, genus Avibirnavirus, family Birnaviridae, using the surrogate Bovine Enterovirus type 1 (Bovine Enterovirus type 1): consult.
- Basic price for activity against Fowlpox virus, using the surrogate Poxvirus Vaccinia (Poxviridae): consult.
- Basic price for activity against bacteriophages (bacteriophages of Lactobacillus lactis, e. gr. P001, P008, ..) (each one): consult.