ASTM E2197-17e1. Standard Quantitative Disk Carrier Test Method for Determining the Bactericidal, Virucidal, Fungicidal, Mycobactericidal and Sporicidal Activities of Liquid Chemical Germicides.
Test accredited by ENAC (National Accreditation Entity).
This test is designed to evaluate the ability of chemical agents to inactivate vegetative bacteria, viruses, fungi, mycobacteria and bacterial spores, on polished stainless-steel disc supports, which represents a hard, non-porous surface environment.
The product to be tested is evaluated by inoculating at least five support discs (for each combination of test substance that you wish to analyze), with one or more of the following test organisms depending on the type of activity you wish to evaluate: Staphylococcus aureus, Pseudomonas aeruginosa, conidia of Trichophyton mentagrophytes, Candida albicans, conidia of Aspergillus niger, Mycobacterium terrae, spores of Bacillus subtilis, and spores of Clostridium sporogenes. For virucidal activity, depending on the claim to be declared and the regulatory agency, it may be necessary to test two or more of the following viruses: Human Adenovirus, Canine Parvovirus, Hepatitis A Virus, Feline Calicivirus, Human Rhinovirus, Human Rotavirus and Human norovirus. After drying the carriers inoculated with the test organism and the interfering substance (a mixture of tryptone, bovine serum albumin and mucin), they are treated with the product. Contact time and temperature can vary as required. The control supports are treated in the same way, but instead of the test product, a liquid that is not harmful to the test organism is added. In this method the evaluation of the chemical agents in wet wipes is not allowed since the test does not include any cleaning or rubbing action. The contact time, the temperature and the concentration of use, or indications on how to apply the product, should be indicated by the client.
After contact time with the disinfectant, the microorganisms/viruses are recovered from the test carriers in eluent and the viable microorganisms are counted or virus titer determined. The results obtained in the plate counts (CFU/carrier), or the virus titer calculated by observing the cytopathic effects in cell cultures inoculated with the eluate and its dilutions, are averaged and converted to log10, thus determining the log10 of the reductions in the test, comparing the carriers treated with the test product and the control carriers.