Instituto Valenciano de Microbiología

Masía El Romeral
Ctra. de Bétera a San Antonio Km. 0.3
46117 Bétera (Valencia)
Phone. 96 169 17 02
Fax 96 169 16 37
CIF B-96337217

Bacillus clausii. Culture and identification; Molecular diagnosis (PCR).

Probiotics are live microbes which when administered in adequate amounts confer a benefit to the host. Probiotics can be dispensed as single microorganism (p. Eg. Lactobacuillus spp., Bacillus clausii, ..), or as a mixture of several strains of Lactobacillus (L. acidophilus, L. sporogenes, L. lactis, L. reuteri, L . plantarum, ...), Bifidobacterium (B. bificum, B. longum, B. infantis, ...), Streptococcus (S. thermophilus, S. lactis, S. faecalis ...), Saccharomyces boulardii, etc. Some of these bacteria are common inhabitants of the human intestine, colonize colon in general. Serious illness and its treatment, develop a hostile environment that alters the normal microflora, encouraging the growth of pathogenic bacteria. Probiotic treatment is to reduce or eliminate potential pathogens and their toxins, providing nutrients, growth factors, stimulating intestinal motility modulating the natural defense mechanisms through normalizing the disturbed intestinal flora, and stimulating Th1 immune response and inhibiting Th2 immune response.
It has been shown that the use of probiotics is effective in preventing antibiotic - associated diarrhea. However, probiotics in use do not provide quick profits, and need prolonged administration for clinical benefits are obtained, such as reducing infection rates, pictures of severe sepsis, reduced ICU stay, reduced number of days of assisted ventilation and mortality in surgical or severe trauma patients. The absence of the beneficial effects required in severe patients undergoing antibiotic treatment, may be because the strains used are not properly adhere to the intestinal mucosa quickly, or that a higher dose is not administered.
Usually, there are some foods such as probiotics in yogurts and other dairy products. To be effective probiotics must pass through the gastric barrier to proliferate and colonize the intestine, even in the presence of bile salts and antibiotics could be administered to that patient. Once in the intestine, must adhere to the cells of the intestinal mucosa, they must reduce the permeability of the mucosa to prevent them from passing pathogenic bacteria from the intestinal lumen to the lymph or blood vessels, and must enhance the local immune response, with immunoglobulin secretion A. it has been shown that probiotics stimulate the immune system, promoting Th1 and T regulatory response, providing IL12, IFN-gamma, IL-10, TGF-beta, and decreasing the Th2 response by inhibiting the release of IL -4.
Probiotics are non - pathogenic microorganisms, so its ingestion is not associated with undesirable effects. Bacillus clausii, one of the bacteria considered probiotic bacterium is an aerobic, spore - forming, capable of surviving passage through the acidic stomach environment and colonize the gut in the presence of antibiotics. There is available in vials with 2 trillion spores / 5 mL (Enterogermina), and typically two vials administered daily for 4 weeks.
This bacterium has been used for the treatment of side effects caused by antimicrobial therapy of some long - lasting infections, in which side effects (nausea, diarrhea, epigastric pain) Helicobacter pylori may occur as in the treatment of infection increasing tolerance erradicativa treatment of this infection, and having been found not affect the rate of elimination, so their use can be beneficial in patients with recurrent infections by H. pylori. It has also been used to prevent recurrent respiratory infections in children with asthma, and reducing the incidence of other infections.

Tests in IVAMI:

  • Conventional culture on selective inhibitors antibiotics normal flora, with identification means.
  • PCR qualitative erm34 with target gene (methylase).

Recommended sample:

Suitable for the product or the location where it is intended to detect.
Feces should have been given to patients.

Preservation and shipment of sample:

Refrigerated (preferred) for less than 2 days.
Frozen: over 2 days.

Delivery term

  • Conventional culture on selective media, identifying: 4 working days.
  • PCR qualitative: 48 hours.

Cost of the test: