Instituto Valenciano de Microbiología
(IVAMI)

Masía El Romeral
Ctra. de Bétera a San Antonio Km. 0.3
46117 Bétera (Valencia)
Phone. 96 169 17 02
Fax 96 169 16 37
Email: 
www.ivami.com
CIF B-96337217

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Cosmetics testing - Microbiology of cosmetics. Detection of Escherichia coli
(UNE-EN ISO 21150: 2016)
 
Test accredited by ENAC (National Accreditation Entity).
 
The test provides information about the presence, or absence, of Escherichia coli in 1 g or 1 mL of the cosmetic sample. To perform the test, the cosmetic is diluted in the ratio 1:10 in an enrichment broth directly, when it is are miscible in water. When the cosmetic is not miscible in water is premixed with a solubilizing agent (polysorbate 80) and subsequently added to the enrichment broth. The enrichment broth should contain a neutralizer when the cosmetic tested contains any antimicrobial agent as preservatives. Neutralizers used must have been previously validated by a validation test. The presence or absence of Escherichia coli in the cosmetic is determined after incubation for 24 to 72 hours at 32.5ºC in the enrichment broth, and reseeding on a selective and differential medium. If colonies compatible with Escherichia coli are observed they are identified by conventional biochemical identification tests and reseeding on selective agar for confirmation.