Instituto Valenciano de Microbiología

Masía El Romeral
Ctra. de Bétera a San Antonio Km. 0.3
46117 Bétera (Valencia)
Phone. 96 169 17 02
Fax 96 169 16 37
CIF B-96337217


Poliovirus types 1, 2 and 3 - Neutralization test in cell culture for detection and titration of antibodies against Poliovirus types 1, 2 and/or 3

Test accredited by ENAC (National Accreditation Entity).

This test uses titrated suspensions of Poliovirus 1, 2 and/or 3 to have the 100 TCID50 units used in the test. The virus suspensions are faced with dilutions of the immunoglobulins product, and after appropriate incubation, the mixtures of 100 TCID50 of virus and dilution of the immunoglobulins product are inoculated in cell culture by twelveplicates (12 replicates) to determine the neutralizing dilution (titer) of the immunoglobulin prproduct, using the Spearman-Kärber calculation method.