Instituto Valenciano de Microbiología

Masía El Romeral
Ctra. de Bétera a San Antonio Km. 0.3
46117 Bétera (Valencia)
Phone. 96 169 17 02
Fax 96 169 16 37
CIF B-96337217


Measles virus - Neutralization test in cell culture for detection and titration of antibodies against measles virus

Test accredited by ENAC (National Accreditation Entity)

This test uses titrated measles virus suspensions to have 100 TCID50 in the volumen used in the test. The virus suspensions are faced with dilutions of the immunoglobulin product and after appropriate incubation, the mixtures of 100 TCID50 of virus and dilution of the of the immunoglobulin product are inoculated in cell culture by twelveplicate (12 replicates) to determine the neutralizing dilution (titer) of the immunoglobulin product, using the Spearman-Kärber calculation method.