Instituto Valenciano de Microbiología

Masía El Romeral
Ctra. de Bétera a San Antonio Km. 0.3
46117 Bétera (Valencia)
Phone. 96 169 17 02
Fax 96 169 16 37
CIF B-96337217


Influenza virus A - Neutralization test for detection and titration of neutralizing antibodies against Influenza A virus, including Influenza A H1N1pdm09 virus.

This test uses suspensions of Influenza A H1N1pdm09 virus (new virus) titrated to have 100 TCID50 in the volume of viral suspension used in the test. The viral suspensions are faced with dilutions of the immunoglobulin product, and after appropriate incubation, the mixtures of 100 TCID50 of virus and dilution of the immunoglobulin product are inoculated in cell culture by twelveplicate (12 replicates), to determine the dilution of the immunoglobulin product that neutralize (titer) the virus, using the Spearman-Kärber calculation method.