Instituto Valenciano de Microbiología
(IVAMI)

Masía El Romeral
Ctra. de Bétera a San Antonio Km. 0.3
46117 Bétera (Valencia)
Phone. 96 169 17 02
Fax 96 169 16 37
Email: 
www.ivami.com
CIF B-96337217

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Garlic and other Allium species, Virus ..., (garlic, onions, leeks, shallots) (OYDV, LYSV, SYSV, WoYSV, GCLV, SLV, GVX, ShVX, GMBFV): Molecular diagnosis (RT-PCR); Identification (sequencing).

Several species of viruses that can affect species of Allium (garlic, onions, leeks, shallots). Plant infection by these viruses has important repercussions for the production of bulbs is greatly reduced (up to 50 to 88%) even though the infection does not kill the plant.

Infection can occur by more than 8 different viruses that can cause infection in partnership forming a viral complex (Garlic viral complex, viral Complex garlic) that causes the disease called "mosaic of garlic" (Garlic mosaic) in that would involve two or more viruses of the genera Potyvirus, Carlaviruses and / or Allexivirus. This disease is characterized by the yellow striped, diffuse chlorotic spots, twisted leaves and mosaics.

These viruses have an RNA genome and belong to three different genera: Potyvirus, Carlavirus and Allexivirus. Species of these viruses are:

Potyvirus genre:

Carlaviruses genre:

Allexivirus genre:

Recommended diagnostic methods

Methods have been used to detect antigens of such viruses as immunoenzymatic methods of various forms of design (ELISA, Dot ELISA, Blotting Immunoassay), but all generally encounter two problems: limited sensitivity and difficulty have specific antibodies to detect each of these viruses).

For these reasons they are currently imposing molecular methods of RT - PCR to increase the sensitivity and speed.

Tests in IVAMI

samples recommended

Preservation and shipment of samples:

Delivering results:

Cost of the test: