Pseudomonas luteola (before Chryseomonas luteola): Isolation in culture; Molecular identification (PCR and sequencing)

Information 01-12-15.

Pseudomonas luteola, formerly Chryseomonas luteola or CDC group Ve-1, is considered an opportunistic pathogen ubiquitously found in moist environments. It is a strict aerobic gram-negative bacillus, from 0.8 to 2.5 μm, mobile by multiple flagella. In culture colonies produce a yellow-orange pigment. It is a non-fermentative, oxidase negative and catalase positive. Its optimal growth temperature is 30°C. P. luteola grows best in brain-heart infusion agar medium with 5% horse blood, although it also grows well on TSA agar, nutritive agar, MacConkey agar, etc.

It is a saprophyte bacterium that has been isolated from many natural sources sources (water, soil and humid environments). It is considered a saprophyte and commensal microorganism and, only rarely, a human pathogen. As an opportunistic pathogen, it can cause bacteremia, meningitis, peritonitis, endocarditis, postoperative endophthalmitis and ulcer infections, generally related to surgical operations or the use of catheters or prostheses. In this sense, it is considered a nosocomial pathogen. In general, most strains are sensitive to broad-spectrum cephalosporins, aminoglycosides, and fluoroquinolones. However, infections associated with implants are very resistant and infected prostheses should be removed whenever possible.

Tests carried out in IVAMI:

• Isolation in culture.

• Molecular identification (PCR and sequencing).

Recommended sample:

• Any sample (plant, environmental, human). Vegetable or environmental samples: 5 to 10 g.

• Clinical samples: according to type of infection.

Conservation and shipment of the sample:

• Refrigerated (preferred) for less than 2 days.

• Frozen: more than 2 days.

Delivery of results:

• 3 to 4 working days. In case of molecular identification another 3 to 4 additional days.

Cost of the test:

• Isolation in cultivation: Consult ivami@ivami.com.

• Molecular identification (PCR and sequencing): Consult ivami@ivami.com.