Measurement of antiviral activity on plastics and other non-porous surfaces (ISO 21702: 2019).

Recently, porous and non-porous products treated with antivirals have also been commercialized. The determination of the antibacterial activity incorporated in non-porous products or in porous products (textiles) is carried out according to ISO 22196 and ISO 20743, respectively. For antiviral activity incorporated into textile products, there was the ISO 18184: 2014 standard, later replaced by the new version of the standard. The new ISO 21702 standard indicates the method of testing antiviral activity in non-porous products. As the standard itself indicates, the method described is based on the standards for products with antibacterial activity ISO 22196 and for products with antiviral activity ISO 18184. The standard ISO 21702 is intended for application to products such as plastics, coating materials, ceramics, natural and artificial leathers, stainless, rubber, etc., that is, plastics and other non-porous surfaces.

Due to the individual sensitivities of each virus to antivirals, the results obtained with a test virus cannot be applied to other viruses. The standard indicates the use of two viruses, one of them relatively susceptible, Influenza A (H3N2) virus (A/Hong Kong/8/68 H3N2) and another more resistant, Feline Calicivirus. However, the standard itself indicates that other viruses can be used as alternatives by indication of the manufacturers. In our laboratory we can use any of the viruses proposed by other ISO, EN or ASTM standards (see virus list included in this information), or other viruses when they can be obtained from culture collections.

In the tests, pieces of the material with antiviral activity and pieces without antiviral activity (negative controls) are used. These pieces are inoculated on their surface with the viral suspension, covered with a foil, and left to incubate at 25ºC and 90% relative humidity for 24 hours, or shorter up to 24 hours, if requested by the manufacturer. Three (3) pieces should be used in each of the test groups, although more pieces can be used if you want to reduce the variability in the results, especially with products that have little antiviral activity. Controls of both, pieces with antiviral and pieces without antiviral are used in the tests to demonstrate that they have no effect that can reduce the infectivity of the virus or the susceptibility of the cells to infectivity by the virus, and at the same time to immediately quantify the virus deposited as inoculum on them. After the contact time, the deposited viruses are collected and quantified, to determine, where appropriate, the reduction obtained in the pieces with antiviral activity, compared to the pieces without antiviral activity. The results are expressed in titer (quantity) of virus per cm2 of sample tested. The standard does not establish a criterion to consider the effectiveness of the product, which leaves to consensus criterion between the parties involved (manufacturers, users, ...).

The tests are carried out using square pieces of 50 ± 2 mm x 50 ± 2 mm and equal or less than 10 mm thick, both of material with antiviral activity and without antiviral activity (controls). Nine (9) pieces of material with antiviral activity and 12 pieces without antiviral activity (negative controls) are required for each virus chosen. For the main tests they will be carried out using at least three samples of each test material treated. At least twelve samples of the untreated material and nine samples of treated material are required.

Cost of tests with viruses recommended by this and other standard guidelines (ISO, EN, ASTM):

  • Basic price with the two viruses indicated by the ISO 18184 standard (Influenza A H3N2 virus -A/Hong Kong/8/68 H3N2-) and Feline Calicivirus: consult to ivami@ivami.com.
  • Basic price with only one of the viruses indicated by the ISO 18184 standard (Influenza A H3N2 virus -A/Hong Kong/8/68 H3N2- or Feline Calicivirus: consult to ivami@ivami.com.
  • Basic Price with the three viruses obligates by the EN 14476 standard for general virucidal activity (Poliovirus type 1, Adenovirus type 5 and Murine Norovirus): consult to ivami@ivami.com.
  • Basic price with only two viruses (any of the three indicated as obligates by the EN 14476 standard for general virucidal activity): consult to ivami@ivami.com.
  • Basic price with only two viruses (Adenovirus type 5 and Murine Norovirus) for Limited virucidal activity according to EN 14476 standard (Activity against Adenovirus, Norovirus , Rotavirus and enveloped viruses): consult to ivami@ivami.com.
  • Basic price with one virus (any of the three indicated as obligate by the EN 14476 for general virucidal activity): consult to ivami@ivami.com.
  • Basic price with Vaccinia virus for limited activity against enveloped viruses: consult to ivami@ivami.com.

Cost of test with other viruses not recommended by EN standard guidelines

  • Basic price with Adenovirus type 2: consult to ivami@ivami.com.
  • Basic price with Canine Distemper virus, family Paramyxoviridae, Genus Morbillivirus) using the surrogate measles virus (family Paramyxoviridae, Genus Morbillivirus): consult to ivami@ivami.com.
  • Basic price with Enterovirus 71: consult to ivami@ivami.com.
  • Basic price with Hepatitis A virus, HM-175 strain: consult to ivami@ivami.com.
  • Basic price with Hepatitis B virus (HBV) with the Pseudorabies virus –PRV- as surrogate of HBV: consult to ivami@ivami.com.
  • Basic Price for Hepatitis C virus (HCV) with the surrogate Bovine Viral Diarrheal virus -BVDV-: consult to ivami@ivami.com.
  • Basic price with Herpes simplex virus (HSV): consult to ivami@ivami.com.
  • Basic price with Human Coronavirus humano 229E: consult to ivami@ivami.com.
  • Basic price with Human Immunodeficiency virus –HIV-, strain RF: consult to ivami@ivami.com.
  • Basic price with Influenza A H1N1 virus (or other available strain: H1N1pdm; H3N2; H5N1): consult to ivami@ivami.com.
  • Basic price with Influenza B virus: consult to ivami@ivami.com.
  • Basic price with Measles virus: consult to ivami@ivami.com.
  • Basic price with Mumps virus: consult to ivami@ivami.com.
  • Basic Price with Papillomavirus (VPH) using the surrogate BK virus (Polyomavirus): consult to ivami@ivami.com.
  • Basic Price with Papovavirus SV40, using the surrogate BK virus (Polyomavirus): consult to ivami@ivami.com.
  • Basic price with Porcine Infuenza virus (Swine Influenza virus): consult to ivami@ivami.com.
  • Basic price with Respiratory Syncytial Virus (RSV): consult to ivami@ivami.com.
  • Basic price with Rotavirus: consult to ivami@ivami.com.