Clinical Microbiology
- Submission form to order Clinical Microbiology tests.
- Samples for Clinical Microbiology testing.
- Acanthamoeba spp. - Amoebic keratitis and granulomatous amoebic Encephalitis (GAE): Microscopic exam; Isolation in culture; Molecular diagnosis (Real-time PCR); Molecular species identification (sequencing).
- Acanthocheilonema reconditum - Molecular diagnosis (PCR).
- Acari human parasites (See Demodex folliculorum and/or Sarcoptes scabiei).
- Achromobacter spp. y Achromobacter xylosoxidans: Molecular diagnosis (PCR); Molecular species identification (sequencing)..
- Acid-fast bacilli (AFB) - (see Mycobacteria).
- Actinobacillus pleuropneumoniae - Molecular diagnosis (PCR).
- Actinomyces israelii - Molecular diagnosis (PCR).
- Actinomyces naeslundii - Molecular diagnosis (PCR).
- Actinomyces spp.: Molecular diagnosis (PCR); Molecular species identification (sequencing)..
- Actinomyces viscosus - Molecular diagnosis (PCR).
- Actopobium spp.: Molecular diagnosis (PCR); Molecular species identification (sequencing).
- Adenovirus (Enteric Adenovirus: serotypes 40 and 41) - Antigen in stools.
- Adenovirus, Influenza, Parainfluenza and Respiratory Syncytial Virus (RSV) - (see Respiratory viruses).
- Aerobic bacteria: Isolation in aerobic culture; Molecular identification (sequencing); Antimicrobial susceptibility tests.
- Aeromonas spp. - Emerging pathogens associated with gastrointestinal and extraintestinal diseases: Isolation in culture; Molecular diagnosis (PCR); Molecular species identification (sequencing).
- African tick bites fever (ATBF) by Rickettsia africae - (see Rickettsia spp.).
- Agglutination - Brucella spp. and SAlmonella spp. (see Brucella spp.; or Salmonella spp.
- Aichivirus - (see Picornaviridae - Picornavirus).
- Alicyclobacillus spp.; Isolation in culture; Molecular diagnosis (PCR); Molecular species identification (sequencing).
- Alphavirus (Arbovirus, Togaviridae): Molecular diagnosis of Alphavirus (RT-PCR); Molecular species identification (sequencing).
- Amebiasis - (see Entamoeba histolytica, Entamoeba dispar and Entamoeba moshkovskii).
- Aminoglycosides resistance ((aac(3)-I; aac(3)-II; aac(3)-II/VI; aac(6´)-Ib; aac(6´)-II; aac(6)-II/Ib; ant(2´´)-Ia; ant(3´´)-Ia; aph(3´)-IIa; aph(3´)-VI; rmtA(3); rmtB; rmtC; rmtD; arm; nmpA genes)
- Anaerobic obligate bacteria (estrict anaerobic bacteria): Isolation in culture (see: Cultures).
- Anaerobic strict bacteria: Isolation in anaerobic culture; Molecular identification (sequencing); Antimicrobial susceptibility tests.
- Anaplasma phagocytophilum (Formerly Ehrlichia phagocytophila - Human Anaplasmosis (Human granulocytic anaplasmosis, Tick-borne fever; Pasture fever) - Microscopic exam; Molecular diagnosis (PCR).
- Ancylostoma spp. (A. caninum, A. braziliensis, A. ceylanicum, A. duodenales, A. tubaeforme) (Necator americanus): Molecular diagnosis (PCR); Molecular species identification (sequencing).
- Anellovirus II (Torque Teno -TTV- virus, virus TTMDV - Torque Teno Midi and TTMV - Mini Torque Teno virus -) - Molecular diagnosis (PCR); Identification by sequencing.
- Angiostrongylus cantonensis, Angiostrongylus vasorum: Molecular diagnosis (PCR).
- Anisakis spp. - Macroscopic and microscopic exam; Molecular identification of species by sequencing.
- Anogenital warts (Papillomavirus -HPV: Human Papillomavirus): Molecular diagnosis (PCR)
- Antibiogram for usual and special bacteria - Disc difusion; Agar dilution MIC; Microdilution MIC.
- Antibiotics - Potency quantification of antibiotics : Turbidimetric (tube) method or diffusion (cylinder-plate or plate) method, according to USP (United States Pharmacopeia).
- Antifungigram - (see Fungi).
- Antimicrobials - Phenotypic and genotypic resistance tests (any in vitro growing bacteria and any available antibimicrobial).
- Aphthovirus - (see Picornaviridae - Picornavirus).
- Arbovirus (Arthropod Borne Virus) - Molecular diagnosis (RT-PCR and sequencing); Type specific antibodies by neutralization for Chikungunya virus, Mayaro, Zika, West Nile (for others consult).
- Arcanobacterium haemolyticum (formerly Corynebacterium haemolyticum): Culture; Molecular diagnosis (PCR).
- Arcobacter spp.: Interest for human and animal pathology and in contamination sources (water and food from animal origin) - Culture; Molecular diagnosis (PCR and sequencing).
- Arenavirus: see Lymphocytic Choriomeningitis virus -LCMV-.
- Arthropods: Molecular identification (sequencing).
- Ascaris lumbricoides - Microscopic exam for eggs in stools IgG antibodies.
- Aseptic meningitis - Enterovirus; Herpes simplex virus type 1 and 2; Varicella-Zoster virus; Lymphocytic choriomeningitis virus; JC virus; Arbovirus; Leptospira interrogans - (see each etiological agent).
- Asian Taenia - Molecular diagnosis (PCR).
- Aspergillus spp. (A. fumigatus, A. flavus, A. terreus, A. glaucus): Galactomannan antigen; Isolation in culture; Molecular diagnosis (PCR); Molecular species identification (sequencing); Genetic comparative typing (microsatellites; RA
- Astrovirus - Molecular diagnosis (RT-PCR).
- Atopobium vaginae: Molecular diagnosis (PCR).
- Atypical Respiratory Serology - Mycoplasma pneumoniae, Chlamydophila pneumoniae, Coxiella burnetii and Legionella pneumophila (see in each agent).
- Aura virus: (see Alphavirus, Togaviridae).
- Avian influenza A - (see Influenza A H5N1 and H7N9).
- Babauki, Virus ..., (Alphavirus, Togaviridae) - (see Arbovirus).
- Babesia spp. (Babesia microti, B. bovis, B. divergens): Microscopic exam; Molecular diagnosis (PCR); Molecular species identification (sequencing).
- Bacillus anthracis - Culture; Molecular diagnosis (PCR).
- Bacillus cereus: Isolation in culture; Molecular identification (sequencing); Molecular diagnosis of toxin genes (emetic toxin-cerulide (gen ces); cytotoxin K (gen cytK), Hemolytic toxin HBL (gen hblc); enterotoxin FM (gen EntFM); enterotoxin T (gen
- Bacillus claussi: Isolation in culture; Molecular identification (sequencing); Molecular diagnosis (PCR).
- Bacillus subtilis (group of Bacillus subtilis species complex): Isolation in culture; Molecular species identification (sequencing); Toxin detection.
- Bacterial and fungal infections in samples without normal flora – Rapid diagnosis through 16S rRNA or 18S-ITS1 genes and ITS2 genes, respectively: Molecular diagnosis (PCR and sequencing).
- Bacterial arthritis - Etiology: Culture; Molecular diagnosis (PCR and sequencing).
- Bacterial meningitis in adults over 50 years and in immunocompromised - Neisseria meningitidis, Streptococcus pneumoniae, Listeria monocytogenes and gram-negative bacilli.
- Bacterial meningitis in adults under 50 years - Neisseria meningitidis and Streptococcus pneumoniae.
- Bacterial meningitis in children - Neisseria meningitidis, Streptococcus pneumoniae and Haemophilus influenzae type b.
- Bacterial meningitis in head trauma, drainage reservoir, immunosuppressed ..., - (Staphylococcus spp, Pseudomonas spp, Gram-negative bacilli, Propionibacterium acnes, coryneform bacteria, ....
- Bacterial meningitis in preterm born - Streptococcus agalactiae (group B), Escherichia coli K1 and Listeria monocytogenes.
- Bacterias, without specification: Isolation in aerobic and anaerobic culture; Molecular identification (sequencing); Antimicrobial susceptibility tests.
- Bacteroides fragilis: Molecular diagnosis of enterotoxin production (PCR).
- Baker-Rosenbach Erysipeloid - (see Erysipelothrix rhusiopathiae).
- Balamuthia mandrillaris (Granulomatous amoebic encephalitis): Molecular diagnosis (PCR).
- Barmah Forest Virus (BFV) (Alphavirus, Togaviridae) - (see Arbovirus).
- Bartonella henselae - IgG antibodies; IgM antibodies; Molecular diagnosis (PCR).
- Bartonella quintana: IgM and IgG antibodies; Molecular diagnosis (PCR).
- Bartonella spp. (B henselae, B. quintana, B. clarridgiae, B. elisabethae, B. vinsonii, B. bacilliformis): Molecular diagnosis (Real-time PCR); Species molecular identification (sequencing).
- Bebaru Virus: see Alphavirus, Togaviridae.
- Beta-lactamases (Chromosomal AmpC; Extended Spectrum ESBLs; A, B, C and D classes Carbapenemases; Metallobetalactamases) - Molecular diagnosis (PCR) and typing by sequencing.
- BK virus (Polyomavirus): Isolation in cell culture; Molecular diagnosis (PCR).
- Blastocystis spp., Blastocystis hominis: Microscopic exam; Culture; Molecular diagnosis (PCR); Subtypes identification (PCR and sequencing).
- Blastomyces dermatitidis: Isolation in culture; Molecular diagnosis (PCR).
- Blood culture.
- Bocavirus: Molecular diagnosis (rt-PCR).
- Body odor, sweating and bacteria involved.
- Bordetella holmesii: Molecular diagnosis (PCR).
- Bordetella parapertussis: IgM and IgG antibodies; Molecular diagnosis (PCR).
- Bordetella pertussis: IgA, IgM and IgG antibodies; Molecular diagnosis (PCR).
- Bornavirus: Neurotropic virus with interest for human and animal pathology:- Molecular diagnosis (PCR).
- Borrelia burgdorferi sensu stricto and B. burgdorferi sensu lato (Lyme disease) - IgG and IgM antibodies; IgG and IgM specific antibodies (Wester-blot); Molecular diagnosis (PCR and sequencing).
- Borrelia recurrentis (Epidemic Relapsing Fever - Molecular diagnosis (PCR).
- Borrelia spp. Endemic Relapsing fever (B.duttoni, B.hispanica, B.hermsi, B.parkeri, B.venezualensis, B.persica, B.turicatae, B.caucasica, B.mazzoti, B.crucidarae,... ) - Molecular diagnosis (PCR and sequencing).
- Botulism - (see Clostridium botulinum in the Clostridium botulinum especific Area.
- Brachyspira spp. - Human intestinal spyrochaetosis: Molecular diagnosis (PCR).
- Brucella spp. - Total, IgM and IgG antibodies; Complete and incomplete antibodies; Rose Bengal test; Coombs test; Molecular diagnosis (PCR).
- Brugia malayi - (see Filaria - Filariasis).
- Brugia timori - (see Filaria - Filariasis).
- Buggy Creek virus (Alphavirus, Togaviridae) (see Arbovirus).
- Burkholderia cepacia complex: Opportunistic pathogens in patients with cystic fibrosis - Culture; Molecular diagnosis (PCR); Species identification (PCR and sequencing).
- Burkholderia pseudomallei (Melioidosis) - Culture; Molecular diagnosis (PCR).
- Calicivirus (Norwalk and related viruses) - Molecular diagnosis (RT-PCR); Identification by sequencing.
- California Encephalitis virus (Bunyavirus, Bunyaviridae) - (see Arbovirus).
- Campylobacter jejuni, C. laridis, C. coli - Culture; Molecular diagnosis (PCR).
- Campylobacter rectus - Molecular diagnosis (PCR).
- Candida - Antigen (mannan) Detection ...; Antibodies; Molecular diagnosis (PCR).
- Candida spp. - Genotyping: Genotype determination (A, B, C, D or E); ALT sequences in RPS genomic region; Microsatellites; MLST (Multilocus Sequencing Typing).
- Capillaria philippinensis: Microscopic exam and molecular identification (sequencing); Molecular diagnosis (PCR).
- Capsular antigens from bacteria: Haemophilus influenzae, Streptococcus pneumoniae, Group B Streptococcus and Neisseria meningitidis (A, B, C, W, Y).
- Carbapenemases class A, B, C and D - (see Beta-lactamases).
- Cardiovirus - (see Picornaviridae - Picornavirus).
- Cat scratch disease - (see Bartonella henselae).
- Cervicitis (Neisseria gonorrhoeae, Chlamydia trachomatis, Herpes simplex virus types 1 and 2) - Molecular diagnosis (PCR).
- Chagas´ disease - (see Trypanosoma cruzi).
- Chikungunya Virus (Alphavirus, Togaviridae) (Arbovirus) - IgM and IgG antibodies; Type specific antibodies by neutralization; Molecular diagnosis (RT-PCR and sequencing).
- Chlamydia trachomatis - Culture; Total, IgA, IgG and IgM antibodies; Molecular diagnosis (PCR); Molecular identification of serotypes (PCR and sequencing).
- Chlamydophila pneumoniae - Culture; Total, IgA; IgG and IgM antibodies; Molecular diagnosis (PCR).
- Chlamydophila psittaci - IgM, IgG and total antibodies; Molecular diagnosis (PCR and sequencing).
- Chronic Fatigue Syndrome (CFS) or Myalgic Encephalomyelitis (ME). Consideration on its infectious etiology.
- Chronic Fatigue Syndrome. Relation with mycoplasmas in blood (Mycoplasma pneumoniae, Mycoplasma hominis, Mycoplasma fermentans and Mycoplasma penetrans): Molecular diagnosis (PCR and sequencing).
- Cladosporium spp. - Molecular diagnosis (PCR).
- Clostridium botulinum: Detection of anti-botulinum toxin antibodies in serum or plasma, using neutralization reference test in mice.
- Clostridium botulinum - Culture; Detection of toxin; Detection of antitoxin A, B and C; Molecular detection of toxin genes by PCR; Bacterial load by Real-Time PCR (see specific area of Clostridium botulinum.
- Clostridium difficile - Culture; Cytotoxin detection in cell culture; Toxin genes A, B and binary toxin (PCR); Gene deletions tcdC (hypervirulent strains); Ribotyping (hypervirulent strains NAP1/BI/027 and others).
- Clostridium perfringens: Interest in human and animal pathology - Culture; Molecular diagnosis (PCR); Identification (PCR and sequencing); Molecular diagnosis of enterotoxin, phospholipase C, beta, epsilon, and iota codifying genes (PCR).
- Clostridium tetani - Toxin detection (experimental inoculation and neutralization test); IgG antidodies; Molecular diagnosis (PCR and sequencing).
- CMV - (see Cytomegalovirus).
- Coccidioides immitis - Molecular diagnosis (PCR).
- Colorado Tick Fever Virus (Orbivirus) - (see Arbovirus).
- Comparison of microorganisms - Molecular comparison of bacteria and fungi using 6 to 8 loci.
- Coombs test - (see Brucella).
- Coproculture - (see Stool cultures).
- Coronavirus MERS (Middle East Respiratory Syndrome) - Molecular diagnosis (RT-PCR and sequencing).
- Coronavirus SARS-CoV-2 (COVID-19 Coronavirus) - Neutralizing protecting SARS-CoV-2 (COVID-19) specific anibodies: neutralizing antibodies by microneutralization test.
- Coronavirus SARS-CoV-2 (COVID-19; 2019nCoV; Wuhan Coronavirus): Molecular diagnosis (Real time RT-PCR).
- Corynebacteria - (see Corynebacterium).
- Corynebacterium diphtheriae - Culture; Detection of toxigenic strains; IgG antibodies; Antitoxin antibodies (neutralization test in guinea pigs); Molecular diagnosis (PCR).
- Corynebacterium spp. (C. jeikeium, C. urealyticum, C. striatum, C. pseudotuberculosis, C. pyogenes, C. pseudodiphtheriticum, etc ...,) - Culture; Antimicrobial susceptibility; Molecular diagnosis (PCR).
- Cosavirus - (see Picornaviridae - Picornavirus).
- Cowpox (CPXV) - (see Poxvirus of animal origin).
- Coxiella burnetii (Q fever): Interest in human and animal pathology and in environmental contamination - IgM, IgA, IgG and total antibodies (ELISA); Phase I and II antibodies (Immunofluorescence); Molecular diagnosis (PCR).
- Coxsackie A (currently types of Enterovirus A species or Enterovirus B species) - (see Picornaviridae - Picornavirus).
- Coxsackie B (currently types of Enterovirus B species) - (see Picornaviridae - Picornaviruses).
- Coxsackievirus (currently types of Enterovirus) - (see Picornaviridae - Picornavirus).
- Crimean-Congo Hemorrhagic Fever (CCHF) - Molecular diagnosis (RT-PCR).
- Cultures - Any kind of culture for bacteria, fungi, viruses, or protozoa that can grow in culture.
- Cryptococcus neoformans - Microscopic exam; Antigen (qualitative or quantification); Culture; Molecular diagnosis (PCR).
- Cryptosporidium spp. - Microscopic exam; Stool antigen; Molecular diagnosis (PCR).
- Cutaneous Larva Migrans (see Ancylostoma caninum and Ancylostoma braziliensis in the Molecular Veterinary Microbiology Area).
- Cyclospora cayetanensis - Molecular diagnosis (PCR) (see Cyclospora.cayetanensis in the Plant Microbiology area).
- Cysticercosis - (see Taenia solium).
- Cytomegalovirus - Antigenemia; Pp65 antigen; Culture (shell vial), IgM and IgG antibodies; Molecular diagnosis (PCR); Viral load; Resistance mutations (PCR and sequencing).
- Dengue Virus (seotypes 1 to 4) - IgG and IgM antibodies; Molecular diagnosis (RT-PCR); Serotypes identification (RT-PCR and sequencing).
- Dermatophytes fungi - Microscopic exam; Culture; Species identification (sequencing); Molecular diagnosis (PCR).
- Dientamoeba fragilis - Molecular diagnosis (PCR).
- Diphyllobothrium spp. (Diphyllobothriasis): Pathogenesis and species - Parasitological diagnosis; Molecular identification of species.
- Dirofilaria repens - (see Filaria - Filariasis).
- Donovanosis / Granuloma inguinale (Klebsiella granulomatis, previously Calymmatobacterium granulomatis) - Molecular diagnosis (PCR).
- Eastern Equine Encephalitis Virus (EEEV) (Alphavirus, Togaviridae) - (see Arbovirus).
- Ebola Virus - Molecular diagnosis (RT-PCR and sequencing).
- EBV - (see Epstein-Barr virus).
- Echinococcus granulosus: Etiological agent of echinococcosis or cystic hydatidosis - IgG antibodies; Molecular diagnosis (PCR).
- Echinococcus multilocularis (Alveolar Hydatidosis) - Molecular diagnosis (PCR).
- Echovirus (currently types of Enterovirus B species) - (see Picornaviridae - Picornaviruses).
- Ehrlichia spp.: Ehrlichia ewingii(granulocytic ehrlichiosis);Ehrlichia phagocytophila(granulocytic ehrlichiosis); Ehrlichia chaffeensis(monocytic ehrlichiosis)-Microscopic exam; Molecular diagnosis (PCR).
- Encephalitozoon cuniculi, Encephalitozoon hellen, Encephalitozoon intestinalis (see Encephalitozoon cuniculi in the Molecular Veterinary Microbiology Area).
- Encephalitozoon cuniculi, Encephalitozoon intestinalis and Enterocytozoon bieneusi (see Microsporidium - Microsporidia..
- Encephalitozoonosis - (see Encephalitozoon cuniculi in the Molecular Veterinary Microbiology Area).
- Encephalomyocarditis virus (see Picornaviridae - Picornavirus) .
- Endemic relapsing fever transmitted by soft ticks (Ornithodorus spp.) - (see Borrelia spp. producing endemic relapsing fever).
- Endotoxins - Quantitative Chromogenic LAL Test.
- Entamoeba dispar - (see Entamoeba histolytica, Entamoeba dispar and Entamoeba moshkovskii).
- Entamoeba histolytica, Entamoeba dispar and Entamoeba moshkovskii - IgG antibodies; Molecular diagnosis (PCR); Species identification (PCR and sequencing).
- Enterobius vermicularis - Microscopic exam; Molecular diagnosis (PCR).
- Enterocytozoon bieneusi (Microsporidiosis) - (see Enterocytozoon bieneusi in the Molecular Veterinary Microbiology Area).
- Enterocytozoon bieneusi (See Microsporidium - Microsporidia).
- Enterovirus (currently types of Enterovirus A, B, D or J species) - (See Picornaviridae - Picornavirus).
- Epidemic Relapsing Fever (Lice-borne) - (see Borrelia recurrentis).
- Epididymitis (Neisseria gonorrhoeae, Chlamydia trachomatis) - Molecular detection (PCR).
- Epstein-Barr virus - Anti-EA IgG and IgM antibodies; Anti-EBNA IgG, IgM; Anti-VCA IgG, IgM; Heterophile antibodies; IgG avidity antibodies to VCA; Molecular diagnosis (PCR); Viral load; EBV types 1 and 2.
- Erysipelothrix rhusiopathiae (Baker-Rosenbach erysipeloid; Pig erysipela; Swine erysipela): Culture; Molecular diagnosis (PCR)
- Erythrovirus B19 - (see Parvovirus B19).
- Enteroaggregative Escherichia coli (EAggEC) - Molecular diagnosis (PCR).
- Enterohemorrhagic Escherichia coli (O157: H7 and others) - Culture; Molecular diagnosis (stx1 and stx2 genes).
- Enteroinvasive Escherichia coli - Culture; Cell invasion; Molecular diagnosis (PCR).
- Enterotoxigenic Escherichia coli - Molecular diagnosis for thermolabile and thermostable toxin (PCR).
- Escherichia coli k1 - Molecular diagnosis (PCR).
- Escherichia coli - Main enteropathogenic virotypes (ETEC, EPEC, EaggEC-EAEC, EIEC, EHEC and DAEC; Escherichia coli K1: Molecular diagnosis (PCR).
- Escherichia coli producing shigatoxin (STEC) - Determination of serogroup-O and Serotype-H: Molecular identification (PCR).
- Escherichia coli: Resistance mutations to antimicrobials (β-lactams, quinolones, TMP-SMX, chloramphenicol, tetracyclines, ...)
- Fasciola hepatica - Antibodies; Molecular diagnosis (PCR).
- Filaria - Filariasis: Clinical syndromes, Species and diagnostic tests - Microscopic exam; Total antibody (antigen of Acanthocheilonema vitae); Molecular diagnostics (PCR), Species identification (PCR and sequencing).
- Flu - (see Influenza A, Influenza B or Influenza A and B).
- Flukes - (see Fasciola hepatica).
- Fort Morgan Virus (Alphavirus, Togaviridae) - (see Arbovirus).
- Francisella tularensis (Tularemia) - Culture; Molecular diagnosis (PCR).
- Free-living amoeba (See Acanthamoeba spp. and Naegleria spp.).
- FTA-ABS - (see Treponema pallidum).
- Fungal infections in bone marrow transplant patients - Etiology: Conventional microbiological tests; Molecular diagnosis (PCR); Species identification (sequencing).
- Fungal meningitis / meningoencephalitis: Cryptococcus neoformans, Candida spp. and in cases of exotic mycosis (Histoplasma, Coccidioides, Blastomyces).
- Fungi - Microscopic exam; Culture; Antifungigram for yeast and filamentous fungi; Molecular diagnosis (PCR); Identification by sequencing.
- Fusarium spp. (F. solani, F. oxysporum, F. verticilloides, F. chlamydosporum, F. sacchari, and other species): Isolation in culture; Molecular diagnosis (PCR); Species molecular identification (sequencing).
- Galactomannan antigen: (see Aspergillus spp.).
- Gardnerella vaginalis - Culture; Molecular diagnosis (PCR).
- Genital Mycoplasma spp.(Mycoplasma hominis, Mycoplasma genitalium, Ureaplasma parvum and Ureaplasma urealyticum) - Culture; Molecular diagnosis (PCR); Identification of species (sequencing).
- Genital ulcers (Treponema pallidum, Haemophilus ducreyi, Chlamydia trachomatis LGV types 1, 2 and 3) - Molecular diagnosis (PCR).
- Genitourinary mycoplasmas (Mycoplasma hominis, Mycoplasma genitalium, Ureaplasma urealyticum, Ureaplasma parvum) - Antimicrobial resistance: Molecular identification (PCR and sequencing).
- Genotypic resistance to antimicrobials - Any antimicrobial (PCR and sequencing).
- Getah Virus (Alphavirus, Togaviridae) - (see Arbovirus).
- Giardia duodenalis (Giardia lamblia) - Microscopic exam; Antigen detection; Molecular diagnosis (PCR).
- Glucan (1-3-β-D-glucan - 1-3) β-D glucan detection.
- Gnathostoma spinigerum, G. hispidum, G. doloresi, G. nipponicum: Molecular diagnosis (PCR).
- Graham technique - Microscopic exam.
- Gram staining - Microscopic exam.
- Group B Streptococcus - (see Streptococcus agalactiae group B).
- HACEK Bacteria: Culture; Molecular diagnosis (PCR)
- Haemophilus ducreyi - Molecular diagnosis (PCR).
- Haemophilus influenzae b - Anti-Haemophilus influenzae serogroup b; Molecular diagnosis (PCR).
- Haemophilus influenzae - Antigen typing (serotyping); Molecular diagnosis (PCR); Molecular antigen typing (PCR and sequencing.
- Halitosis causing bacteria - (see Periodontitis).
- Hantavirus - Molecular diagnosis (RT-PCR).
- Haplorchis taichui (see Opisthorchis spp.).
- Heartworm - (see Filaria - Filariasis).
- Helicobacter pylori: genotypic resistance to clarithromycin, amoxicillin, metronidazole, levofloxacin, tetracyclines and rifabutin - PCR and sequencing.
- Helicobacter pylori: genotyping - PCR and sequencing.
- Helicobacter pylori - Stool antigen; Culture; IgA, IgM and IgG antibodies; Molecular diagnosis (PCR).
- Helminths - Microscopic exam; Macroscopic identification; Molecular identification (PCR and sequencing).
- Hemorrhagic Fevers viruses - (see each etiologic virus).
- Hepatitis A (Hepatovirus) - Total and IgM antibodies; Molecular diagnosis (RT-PCR); Genotyping (PCR and sequencing).
- Hepatitis B - HBs antigen (quantitafication and confirmation); HBeAg; Anti-HBc, anti-HBe and anti-HBc IgM antibodies; Molecular diagnosis (PCR); Viral load; Genotype; Resistance mutations.
- Hepatitis B virus – Drug resistance mutations: Molecular identification (PCR and sequencing).
- Hepatitis C Virus: Comparison of genomic sequences of hepatitis C virus in plasma samples - PCR and sequencing of several genomic regions; Phylogenetic analysis.
- Hepatitis C virus (HCV) in dialysis fluids - Viral load; Molecular diagnosis (rt-RT-PCR).
- Hepatitis C virus - Total and IgM antibodies; Immunoblot (WB); Molecular diagnosis (RT-PCR); Viral load; Genotyping; Q80K polymorphism and IL-8 genetic; Resistance to protease inhibitors (NS3/4A), NS5A inhibitors and polymerase inhibitors (NS3)
- Hepatitis D (delta) virus genotyping: Relationship with the geographical origin and the clinical course of the disease - Antigen; Total and IgM antibodies; Molecular diagnosis (RT-PCR); Genotyping HDV-1 to HDV-8 (RT-PCR and sequencing)
- Hepatitis E virus - IgG and IgM antibodies; Molecular diagnosis (RT-PCR); Genotyping HDV-1 to HDV-8 (RT-PCR and sequencing).
- Hepatitis G virus - Molecular diagnosis 5´-UTR and NS3 region (RT-PCR).
- Hepatovirus - (see Picornaviridae - Picornavirus).
- Herpes simplex 1 and / or 2 - Anti-HSV-1 and HSV-2 IgG and IgM antibodies; Culture; Molecular diagnosis (PCR).
- Herpes simplex virus: Resistance to acyclovir, penciclovir, foscarnet, cidofovir and adefovir (UL23-UL30 -timidinaquinasa and DNA-polymerase- genes) - Genotypic tests (PCR and sequencing) and phenotypic (culture and IC50).
- Herpesvirus type 6 - IgG and IgM antibodies; Molecular diagnosis (PCR); Viral Load.
- Herpesvirus type 6 - Viral load.
- Herpesvirus type 7 - Molecular diagnosis (PCR).
- Heterophile antibodies (mononucleosis) - (see Epstein-Barr Virus).
- HHV-6 - (see Herpesvirus type 6).
- HHV-7 - (see Herpesvirus type 7).
- HHV-8 - (see Herpesvirus type 8).
- Highlands J Virus (Alphavirus, Togaviridae) (see Arbovirus).
- Histoplasma capsulatum - Culture; Molecular diagnosis (PCR).
- HIV-1: Viral RNA and Proviral DNA; Viral load; Total antibodies and WB confirmation; Resistance mutations to transcriptase, protease, integrase and fusion inhibitors; Tropism.
- HIV-2. Genotypic resistance to protease (PI), Nucleoside/Nucleotides reverse transcriptase (NRTIs); Non-nucleosides reverse transcriptase (NNRTIs); Integrase (INSTIs) and Fusion (FIs) inhibitores.
- HIV-2 - Human Immunodeficiency virus type 2 viral load.
- HIV-2 - Viral RNA and Proviral DNA; Antibodies by immunoblot (WB); Genotypic resistance to Reverse transcriptase (NRTI), protease (PI) and Integrase inhibitors.
- Hookworm: see Ancylostoma spp. - Necator americanus.
- HPV - (see Papillomavirus).
- HTLV-III (HTLV III new, different from initial naming of HIV virus) - Molecular diagnosis (RT-PCR).
- Human genetics - Molecular genetic diagnosis tests (see each disease in the specific Human gene mutations Area).
- Human Herpesvirus type 8 (HHV-8) - IgM and IgG antibodies; Molecular diagnosis (PCR).
- Human Papillomavirus (HPV) – Quantification of mRNA expression from E6 and E7 oncogenes: Quantitative real time RT-PCR (RT-rt qPCR).
- Human T cell lymphotropic viruses types 1 and 2 (HTLV-1 and -2) - Antibodies; Antibodies confirmation test; Molecular diagnosis (RT-PCR); Proviral DNA (PCR).
- Hydatidosis - (see Echinococcus granulosus and Echinococcus multilocularis).
- Hymenolepis diminuta, Hymenolepis nana: Microscopic exam; Molecular diagnosis (PCR).
- Identification of microorganisms and organisms: Aerobic or Anaerobic bacteria; Mycobacteria; Fungi; Protozoa; Helminths; Arthropods including insects; Mamifers; Birds, etc. - PCR and sequencing.
- Igbo-Ora Virus (Alphavirus, Togaviridae) - (see Arbovirus).
- Ilheus virus (ILHV) (Flaviviridae, Flaviviridae) - (see Arbovirus).
- infections and diseases caused by pet birds (Zoonoses in pet birds): microorganisms involved, impaired persons and microbiological diagnosis in IVAMI (see each agent in the Molecular Veterinary Microbiology Area).
- Influenza A and B seasonal - Virus culture; Molecular diagnosis (RT-PCR).
- Influenza A (H1N1) pdm09 (new virus) - Culture; Specific type antibodies (Neutralization test); Molecular diagnosis (RT-PCR); Typing by sequencing; Resistance to neuraminidase inhibitors (sequencing).
- Influenza A H5N1 - Molecular diagnosis (RT-PCR).
- Influenza A H7N9 - Molecular diagnosis (RT-PCR).
- Influenza A seasonal (Influenza A H1N1, H3N2, H5N1, H7N9 or others) - IgG, IgA and IgM antibodies; Antigen In respiratory secretions; Culture; Molecular diagnosis (RT-PCR).
- Influenza B seasonal - IgA, IgG and IgM antibodies; Antigen in respiratory secretions; Culture; Molecular diagnosis (RT-PCR).
- Intestinal Protozoa (Giardia lamblia, Cryptosporidium parvum / Cryptosporidium hominis, Entamoeba histolytica, Dientamoeba fragilis) - Combined test by real-time PCR (rt-PCR).
- Isospora belli (Cystoisospora belli - Microscopic exam; Molecular diagnosis (PCR).
- Japanese Encephalitis virus (JEV) (Flavirivirus, Flaviviridae) - (see Arbovirus).
- JC virus (Polyomavirus Progressive multifocal leukoencephalopathy) - Molecular diagnosis (PCR).
- Kingella kingae - Culture; Molecular diagnosis (PCR for toxin).
- Klebsiella granulomatis (Donovanosis) - (see Donovanosis - Granuloma inguinale).
- Klebsiella pneumoniae, magA, rpmA, wzcK1 and wzcK2 genes - Molecular diagnosis (PCR).
- Klebsiella pneumoniae subsp. rhinoscleromatis - Rhinoscleroma etiology - Molecular diagnosis (PCR); Identification (PCR and sequencing).
- Kobuvirus - (see Picornaviridae - Picornavirus).
- Kunjin Virus (KUNV) (Flavirivirus, Flaviviridae) - (see Arbovirus).
- Kyasanur Forest Disease Virus (KFDV) (Flavirivirus, Flaviviridae) - (see Arbovirus).
- Kyzylagach Virus ( Alphavirus, Togaviridae ) - (see Arbovirus).
- LAL (Quantitative chromogenic LAL test) (see Endotoxins).
- Legionella pneumophila - Epidemiological molecular comparison of strains (7 genes: flaA, pilE, asd, mip, mompS, proA and/or neuA): Molecular amplification (PCR) and sequencing.
- Legionella pneumophila - Total, IgG and IgM antibodies; Antigen in urine; Culture from respiratory or environmental samples; Molecular diagnosis (PCR); Identification of other Legionella species.
- Leishmania spp.: Leishmaniosis in humans and dogs – Molecular diagnosis (Real-time PCR); Species identification (PCR and sequencing); Antibodies.
- Leptospira interrogans and its serovars icterohaemorragiae, hebdomadis, autumnalis, canicola, hyos, bataviae javanica, pomona, ballum,grypothyphosa,panama,hardjo,celledoni..)-Culture;MAT antibodies;Molecular diagnosis;Identification
- Listeria monocytogenes - Control of Listeria monocytogenes asymptomatic infection in pregnant women.
- Listeria monocytogenes - Culture; Molecular diagnosis (PCR); Molecular serotyping (1/2a, 1/2b, 1/2c, 3a, 3b, 3c and 4b).
- Liver flukes - (see Fasciola hepatica).
- Loa loa (see Filaria - Filariasis).
- Lues - (see Treponema pallidum).
- Lyme disease - (see Borrelia burgdorferi sensu stricto and Borrelia burgdorferi sensu lato).
- Lymphocytic Choriomeningitis Virus - Molecular diagnosis (RT-PCR).
- Lymphogranuloma venereum: (Chlamydia trachomatis LGV types 1, 2 and 3) - Molecular diagnosis (PCR).
- Malaria - (see Plasmodium spp.).
- Malassezia furfur (Pityriasis versicolor) and Malassezia spp. - Microscopic exam; Culture; Molecular diagnosis (PCR); Identification of species (PCR and sequencing).
- Malassezia pachydermatis and other Malassezia spp.: Antifungal susceptibility testing (see Malassezia pachydermatis and other Malassezia species in the Molecular Veterinary Microbiology Area.
- Mannan antigen: (see Candida spp.).
- Mansonella ozzardi - (see Filaria - Filariasis).
- Mansonella perstans - (see Filaria - Filariasis).
- Mansonella streptocerca - (see Filaria - Filariasis).
- Marburg Virus - Molecular diagnosis (RT-PCR).
- Mayaro Virus (Alphavirus, Togaviridae - (see Arbovirus).
- Measles - Culture; IgG and IgM antibodies; Specific antibodies quantification by neutralization assay; Molecular diagnosis (RT-PCR).
- Measles virus - Identification of wild and vaccine strains and genotyping through H (hemagglutinin) and N (neuraminidase) genes: Molecular identification (sequencing).
- Mediterranean spotted fever - (see Rickettsia spp.)
- Melioidosis - (see Burkholderia pseudomallei).
- Meningitis / viral meningoencephalitis - Enterovirus; Herpes simplex virus types 1 and 2; Varicella-Zoster virus; Lymphocytic choriomeningitis virus; JC virus; Arboviruses.
- Metallo-beta-lactamases - (see Beta-lactamases).
- Metapneumovirus - Molecular diagnosis (RT-PCR).
- Microsporidium - Microsporidia (Encephalitozoon spp., Enterocytozoon bieneusi and other genera): Molecular diagnosis; Species identification (PCR and sequencing).
- Middelburg Virus (Alphavirus,Togaviridae) - (see Arbovirus).
-
Molecular diagnosis (PCR, RT-PCR, real Time PCR
, real time vRT-PCR - (see each microorganism).) - Molluscum contagiosum virus in clinical samples, pool water and other shared water systems - Molecular diagnosis (PCR).
- Monkeypox virus (MPXV) - (see Poxvirus of animal origin).
- Mononucleosis - (see Epstein-Barr virus).
- Morganella morganii - Culture; Identification.
- Mucormycosis - (see Zygomycetes).
- Mumps - (see Mumps virus).
- Mumps virus - IgG and IgM antibodies; Molecular diagnosis (RT-PCR).
- Murray Valley Encephalitis Virus (MUEV) (Flavirivirus, Flaviviridae) - (see Arbovirus).
- Mycobacteria - Microscopic exam; Culture (LJ); MGIT culture system; Conventional susceptibility test for first, second and third line drugs; Molecular diagnosis (PCR); Resistance mutations; Molecular identification.
- Mycobacterium abscessus-Mycobacterium chelonae complex: Molecular diagnosis (PCR); Macrolides resistance mutations -erm(41); rrl- genes; Aminoglycosides resistance mutations -rrs<{/I> gene- (sequencing).
- Mycobacterium avium - (see Mycobacteria).
- Mycobacterium bovis: Molecular diagnosis (PCR).
- Mycobacterium immunogenum (Mycobacteium chelonae Mycobacterium abscessus complex): Implications in the metallurgical and related industries, and hospital infections - Culture;Qualitative or quantitative rt-PCR;Molecular identification
- Mycobacterium leprae (Lepra) - Molecular diagnosis (PCR).
- Mycobacterium marinum - Culture; Molecular diagnosis (PCR).
- Mycobacterium spp. - Molecular diagnosis (PCR).
- Mycobacterium tuberculosis complex - Culture; Molecular diagnosis (PCR); Antimicrobial susceptibility tests; Resistance mutations (amplification and sequencing of rpoB, katG, mabA, embB, pncA, rpsL, rrs, gyrA and tlyA genes).
- Mycoplasma contaminants in cell cultures - Molecular diagnosis (PCR); Molecular identification (PCR and sequencing).
- Mycoplasma fermentans: Respiratory and genitourinary tract infections - Molecular diagnosis (PCR); Species identification (PCR and sequencing).
- Mycoplasma genitalium: Molecular diagnosis (PCR); Resistance to macrolides and quinolones (sequencing).
- Mycoplasma hominis - Molecular diagnosis (PCR).
- Mycoplasma penetrans: Molecular diagnosis (PCR).
- Mycoplasma pneumoniae - IgG and IgM antibodies; Molecular diagnosis (PCR).
- Mycoplasmas - (see species: Mycoplasma genitalium, Mycoplasma hominis, Ureaplasma urealyticum, Ureaplasma parvum, Mycoplasma pneumoniae).
- Naegleria spp. - Molecular diagnosis (PCR).
- Ndumu Virus (Alphavirus, Togaviridae) - (see Arbovirus).
- Neisseria gonorrhoeae - Culture; Molecular diagnosis (PCR).
- Neisseria meningitidis - Anti-Neisseria meningitidis serogroups A + C and serogroup C; Bactericidal antibodies serogroups A, B, C, Y and W135 (SBA: Serum bactericidal Antibody); Molecular diagnosis (PCR).
- Newcastle disease virus - Molecular diagnosis (RT-PCR).
- Nipah virus - Molecular diagnosis (PCR).
- Nocardia - Total antibodies; Culture; Molecular diagnosis (PCR); Identification of species (sequencing).
- Norovirus - Molecular diagnosis (rt-RTPCR); type identification by sequencing (see Norovirus and Sapovirus in the Food Microbiology Area).
- Norwalk and related viruses - (see Norovirus).
- Nucleic acid sequencing - Any application for identification of species of microorganisms; Molecular comparison of microorganisms; Resistance mutations; Mutations of genetic diseases.
- O´Nyong-Nyong Virus (ONNV) (Alphavirus, Togaviridae) - (see Arbovirus).
- Ockelbo virus ( Alphavirus, Togaviridae) - (see Arbovirus).
- Odor feet (smelly feet): bacteria involved and control measures.
- Omsk Hemorrhagic fever virus (OHFV) (Flavirivirus, Flaviviridae) - (see Arbovirus).
- Onchocerca volvulus - (see Filaria - Filariasis).
- Onychomycosis (Tinea unguium) - Etiologic fungi: Culture; Molecular identification (PCR and sequencing).
- Opisthorchis spp. (O viverrini, O. felineus) - Microscopic exam; Molecular diagnosis (PCR); Molecular species identification (PCR and sequencing).
- Orf virus (ORFV) - (see Poxvirus of animal origin).
- Oropuche Virus (Bunyavirus, Bunyaviridae) - (see Arbovirus).
- Papillomavirus - Molecular diagnosis (PCR); Papillomavirus typing (PCR and sequencing.
- Papovavirus SV-40 - Molecular diagnosis (PCR).
- Paracoccidioides brasiliensis - Culture; Molecular diagnosis (PCR).
- Parainfluenza - IgG and IgM antibodies (Parainfluenza 1, 2 and 3); Antigen in respiratory secretions; Viral culture; Molecular diagnosis (RT-PCR).
- Paramyxovirus: Molecular diagnosis; Molecular identification of species (PCR and sequencing).
- Parapoxvirus (see Poxvirus of animal origin).
- Parasitic meningitis / meningoencephalitis - Toxoplasma gondii, Toxocara canis, Neurocysticercosis, free-living amoebae, Angyostrongylus cantonensis, Gnathostoma spinigerum.
- Paravaccinia - (see Poxvirus of animal origin).
- Parechovirus - (see Picornaviridae - Picornavirus).
- Pariodontitis causing bacteria (Aggregatibacter actinomycetemcomitans, Campylobacter rectus, Porphyromonas gingivalis, Prevotella intermedia, Tannerella forsythia, Treponema denticola) - Molecular diagnosis (PCR).
- Parvovirus B19 (Erythrovirus B19) - IgG and IgM antibodies; Molecular diagnosis (PCR).
- Pasteurella spp. (Pasteurella multocida subsp. multocida and other species) - Culture; Molecular diagnosis (PCR).
- PCR - (see each microorganism).
- Pegivirus: Molecular diagnosis (PCR).
- Pelvic inflammatory disease.
- PFGE (Pulse Field Gel electrophoresis): Comparison of strains.
- Picornaviridae (; ... ) - Molecular diagnosis (RT-PCR); Molecular identification.
- Plasmodium spp.: Microscopic exam; Total, IgG and IgM antibodies; Molecular diagnosis (PCR); Molecular species identification and Drug resistance (PCR and sequencing).
- Plesiomonas shigelloides – Culture; Identification; Molecular diagnosis (PCR).
- Pneumocystis jirovecci (previously Pneumocystis carinii) - Molecular diagnosis (PCR); Resistance mutations for the first line treatment (TMP-SMX) (PCR and sequencing).
- Poliovirus 1, 2 and 3 (currently Enterovirus C specie serotypes) - Antibodies by neutralization to Poliovirus 1, 2 and 3; Molecular diagnosis (PCR); Molecular identification (sequencing); Antigenic typing.
- Polymerase Chain Reaction - (see PCR).
- Polyomavirus: See BK virus and JC virus.
- Powasan Encephalitis Virus (PEV) (Flavirivirus, Flaviviridae) - (see Arbovirus).
- Poxvirus of animal origin - Molecular diagnosis (PCR); Molecular identification (PCR and sequencing).
- PRA - (see Mycobacteria, Molecular identification PRA).
- Prions - Protein PrPsc detection.
- Proctitis (Neisseria gonorrhoeae, Chlamydia trachomatis, Ureaplasma urealyticum, Herpes simplex virus types 1 and 2) - Molecular diagnosis (PCR).
- Propionibacterium acnes - Culture; Molecular diagnosis (PCR).
- Prostatitis: etiology and diagnosis - Culture; Identification; Molecular diagnosis (PCR).
- Prototheca spp.: Interest in human and animal pathology and in enviromental contamination sources (environmental samples, foods and animal samples - Culture; Molecular identification (PCR and sequencing) (see in the Water Microbiology Area).
- Protozoa - Microscopic exam; Culture when possible; Molecular diagnosis (PCR).
- Pseudocowpox virus (PCPV) - (see Poxvirus of animal origin).
- Pseudomonas aeruginosa - Culture; Molecular diagnosis (PCR).
- Pustular Bovine Stomatitis virus (BPSV) - (see Animal Poxvirus).
- Q fever - (see Coxiella burnetii).
- Rabies: Diagnosis of infection in any animal or human bites - Virus Isolation (mouse inoculation); Antigen detection (immunofluorescence); Molecular diagnosis (RT-PCR); Specific antibodies (neutralization); Genotyping
- RAPD (Random Amplification of Polymorphic DNA): epidemiological strain comparison.
- Resistance to fluoroquinolones - qnrA, gyrA, parC, marR, acrR genes: Molecular diagnosis (PCR and sequencing).
- Resistance to glycopeptides (vancomycin and teicoplanin) in Enterococcus spp. Phenotypes VanA, VanB, vand, and other less frequent (VanC, C2 / 3, E, F, G, L, M) - Molecular diagnosis (PCR and sequencing).
- Respiratory Adenovirus: Isolation in cell culture; IgM and IgG antibodies (ELISA); Molecular diagnosis (rt-PCR); Molecular species identification (A to B) (sequencing); Type identification (sequencing).
- Respiratory Coronavirus - Molecular diagnosis (RT-PCR).
- Respiratory infections - Antibodies against Adenovirus antibodies, Influenza A and B, Parainflenza 1, 2 and 3; respiratory syncytial virus; Chlamydophila pneumoniae, Chlamydophila psittaci; Coxiella burnetii, Legionella pneumophila; Mycoplasma
- Respiratory Syncytial Virus (RSV) - Total, IgG and IgM antibodies; Antigen in respiratory secretions; Culture; Molecular diagnosis (RT-PCR).
- Respiratory Syncytial Virus type A - Molecular diagnosis (RT-PCR).
- Respiratory viruses in secretions - Detection of viral antigens (Influenza A, Influenza B, Parainfluenza 1, 2 and 3, Adenovirus, Respiratory syncitial virus).
- Rhinovirus (currently Rhinovirus A, B and C species) - Molecular diagnosis (RT-PCR).
- Rickettsia africae - (see Rickettsia spp.).
- Rickettsia conorii - (see Rickettsia spp.).
- Rickettsia spp: Typhus group(R.prowazekii, R.typhi), spotted fevers group( R.conorii, R.africae, R.slovaca, R.rickettsii, R.sibirica, R.japonica, R.australis, ...), pustular typhus(R.akari)-Culture; Molecular diagnosis (
- Rift Valley Fever virus (+/-ssRNA ambisense, -ssRNA segments L y M, +/-ssRNA segment S, Bunyavirales, Phenuiviridae, Phlebovirus): Molecular diagnosis (RT-PCR).
- Rochalimaea - Molecular diagnosis (PCR).
- Rocio virus (ROCV) (Flaviviridae, Flaviviridae) - (see Arbovirus).
- Ross River virus (RRV) (Alphavirus, Togaviridae) - (see Arbovirus).
- Rotavirus - Stool antigen; Molecular diagnosis (RT-PCR).
- RPR - (see Treponema pallidum).
- Rubella - IgG and IgM antibodies; IgG antibodies avidity; Molecular diagnosis (RT-PCR).
- Sagiyama virus (Alphavirus, Togaviridae) - (see Arbovirus).
- Saint Louis Encephalitis virus (SLEV) (Flavirivirus, Flaviviridae) - (see Arbovirus).
- Salmonella spp. - Culture; Molecular diagnosis (PCR); Molecular O and H antigens typing; Antibodies to O and H antigens; Molecular strains comparison.
- Sandfly Naples virus Fever (SNVF) (Phlebovirus, Bunyaviridae) - (see Arbovirus).
- Sandfly Sicilian virus Fever (SFSV) (Phlebovirus, Bunyaviridae) - (see Arboviruses).
- Sandfly Tuscany virus Fever (TOSV) (Phlebovirus, Bunyaviridae) - (see Arbovirus).
- Sapovirus - (see Norovirus and Sapovirus in the Food Microbiology Area).
- Sarcoptes scabiei – Scabies etiological agent: Molecular diagnosis (PCR).
- Schistosoma spp. - (see Schistosomiasis).
- Schistosomiasis (Schistosoma spp.) - IgG antibodies; Molecular diagnosis (PCR); Molecular identification of species (PCR and sequencing).
- Semliki Forest virus (SFV) (Alphavirus, Togaviridae) - (see Arbovirus).
- Serratia marcescens and Serratia spp.: Isolation in culture; Molecular diagnosis (PCR and PCR with sequencing).
- Sexually Transmitted Infections / Diseases - Molecular diagnosis (PCR), according to clinical manifestations.
- Shiga toxin-producing Escherichia coli (STEC) - Serogroups: Molecular identification (PCR).
- Shigella (Shigella dysenteriae, S. sonnei, S. flexneri, S. boydii) - Culture; Molecular diagnosis (PCR); Antigenic typing.
- Sindbis virus (SV) (Alphavirus, Togaviridae) - (see Arbovirus).
- Sporothrix schenckii - Microscopic exam; Culture; Molecular diagnosis (PCR).
- Sporotrichosis - (see Sporothrix schenckii).
- Staphylococcus aureus - Methicillin resistance; SCCmec, SPA, MSLT typing; TSST-1 and exfoliative toxins (PCR).
- Staphylococcus aureus – Panton-Valentine toxin – lukS-PV and lukF-PV genes: Culture; Molecular diagnosis (PCR).
- Staphylococcus pseudointermedius: Molecular diagnosis (PCR).
- Staphylococcus spp., Identification of Coagulase-negative Staphylococcus (S. capitis, S. caprae, S. epidermidis, S. haemolyticus, S. hominis, S. lugdunensis, S. pasteuri, S. saprophyticus, S. warneri, S. xylosus): Molecular dia
- Streptobacillus moniliformis – Rat bite fever; Haverhill fever.
- Streptococcus agalactiae group B: Effect on newborns and carrier status in pregnant women - Culture; Molecular diagnosis (PCR).
- Streptococcus pneumoniae - Specific types antibodies; opsonofagocytic antibodies; Antigen in urine; Molecular diagnosis (PCR); I>erm and mef genes mutations; Capsular antigen typing (PCR; capsule swelling assay -Quellung-).
- Streptococcus pyogenes - Molecular diagnosis (PCR); M protein typing (emm gene; Toxic shock syndrome-like toxin gene; Erythrogenic toxins SpeA, SpeB and SpeC genes.
- Streptococcus pyogenes - Species detection in clinical samples: Culture; Molecular diagnosis (PCR).
- Strongyloides stercoralis (Strongyloidiasis) - Microscopic exam; Stools culture (Harada-Mori method or Baermann method); IgG antibodies; Molecular diagnosis (PCR).
- Strongyloidiasis - (see Strongyloides stercoralis)
- Syphilis - (see Treponema pallidum).
- Taenia saginata - Molecular diagnosis (PCR).
- Taenia solium - IgG antibodies; Molecular diagnosis (PCR).
- Tetanus - (see Clostridium tetani).
- Thelaziosis (Thelazia callipaeda) (See Thelaziosis - Thelazia callipaeda in the Veterinary Molecular Microbiology area).
- Tick-borne encephalitis virus (TBEV) - (see Arbovirus).
- Tick-borne encephalitis virus (TBEV)) (Flavirivirus, Flaviviridae) - (See Arbovirus).
- Toxocariasis (Visceral larva migrans, Ocular larva migrans, Covert toxocariasis) (Toxocara canis and Toxocara cati) - IgG antibodies; Molecular diagnosis (PCR); Molecular identification (PCR and sequencing).
- Toxoplasma gondii - IgG, IgA and IgM antibodies; IgG antibody avidity; Molecular diagnosis (PCR).
- Treponema pallidum - Darkfield microscopic exam; Molecular diagnosis (PCR); Reaginic antibodies (RPR and VDRL); Treponemal FTA-ABS IgG and IgM antibodies;
- Trichinosis - Trichina (Trichinella spp.) - Diagnosis in animals and food (artificial digestion of tissues and trichinoscopy); Species identification (PCR and sequencing); Diagnosis in infected humans.
- Trichomonas vaginalis - Metronidazole (nitroimidazoles) resistance: Molecular identification of resistance mutations (sequencing).
- Trichomonas vaginalis - Microscopic exam; Culture; Molecular diagnosis (PCR).
- Trichuris trichiura: Microscopic exam; Molecular diagnosis (PCR).
- Tropheryma whipplei (Whipple´s disease) - Molecular diagnosis (PCR).
- Tropical pulmonary Eosinophilia - IgG antibodies against filaria; IgG antibodies for differential diagnosis Schistosoma spp., Strongyloides stercoralis, Ascaris lumbricoides, Toxocara spp. and Basiodiobolus ranarum.
- Trypanosoma brucei rhodesiense and T. brucei gambiense - Microscopic exam; Molecular diagnosis (PCR).
- Trypanosoma cruzi (Chagas´ disease): IgG and IgM antibodies; Microscopic exam; Culture; Molecular diagnosis (PCR).
- Trypanosoma rangeli - Molecular diagnosis (PCR); Microscopic exam.
- Tularemia - (see Francisella tularensis).
- Tuscany virus (Phlebovirus, Bunyaviridae) - (see Arbovirus).
- Una virus (UNAV) (Alphavirus, Togaviridae) - (see Arbovirus).
- Ureaplasma parvum - (see Genital Mycoplasma spp.).
- Ureaplasma urealyticum - (see Genital Mycoplasma spp.).
- Urethral or vaginal discharge (Neisseria gonorrhoeae, Chlamydia trachomatis, Ureaplasma urealyticum, Ureaplasma parvum, Candida albicans, Trichomonas vaginalis) - Molecular diagnosis (PCR).
- Urethritis (Neisseria gonorrhoeae, Chlamydia trachomatis, Ureaplasma urealyticum, Ureaplasma parvum, Trichomonas vaginalis, Herpes simplex virus types 1 and 2) - Molecular diagnosis (PCR).
- Vaccinia virus (VACV) - (see Poxvirus of animal origin).
- Vaginitis and vulvovaginitis (Trichomonas vaginalis, Candida albicans) - Molecular diagnosis (PCR).
- Vaginosis (Gardnerella vaginalis, Mobiluncus spp.) - Molecular diagnosis (PCR).
- Varicella-Zoster virus - Culture; Total, IgG and IgM antibodies; IgG antibody avidity; Antigen detection; Molecular diagnosis (PCR).
- Variola: Molecular diagnosis (PCR).
- VDRL - (see Treponema pallidum).
- Venezuelan equine encephalitis virus (VEEV) (Alphavirus, Togaviridae) - (see Arbovirus).
- Vibrio cholerae cholerae classical biotype; Vibrio cholerae El Tor biotype; Serogroups O1 and O139 -Bengal-, and Vibrio cholerae NAG (non-O1, Non-O139) - Identification; toxin production detection.
- Vibrio parahaemolyticus Kanagawa positive: Molecular detection (PCR).
- Visceral Larva migrans (see Toxocariasis: Toxocara canis and Toxocara cati).
- West Nile Fever virus (WNFV) (Flavirivirus, Flaviviridae) - (see Arbovirus).
- West Nile Virus (WNV) (Flavirivirus, Flaviviridae) - (see Arbovirus).
- Western equine encephalitis virus (WEEV) (Alphavirus, Togaviridae) - (see Arbovirus).
- Whataroa virus (Alphavirus, Togaviridae) - (see Arbovirus).
- Whipple´s Disease - (see Tropheryma whipplei).
- Wuchereria bancrofti - (see Filaria - Filariasis).
- XMRV (Xenotropic Murine Leukemia virus-related virus) - Molecular diagnosis (RT-PCR).
- Yellow Fever virus (YFV) (Flavirivirus, Flaviviridae) - (see Arbovirus).
- Yersinia enterocolitica: Culture; Molecular diagnosis (PCR); Molecular identification of pathogenic strains; Molecular identification of the main serotypes.
- Yersinia pestis - Culture; Molecular diagnosis (PCR).
- Yersinia pseudotuberculosis - Molecular diagnosis (PCR).
- Zika virus (ZIKV) (Flavivirus, Flaviviridae) (Arbovirus) - Molecular diagnosis (rt-RT-PCR); Specific antibodies by neutralization test.
- Zygomycetes (Mucorales) -Mucormycosis-: Microscopic exam; Culture; Molecular diagnosis (PCR); Molecular identification (sequencing); Molecular diagnosis for Absidia spp., Cunninghamella spp., Mucor spp., Rhizopus spp., Rhizomucor spp.; Antif