Molluscum contagiosum virus in clinical samples, pool water and other shared water systems - Molecular diagnosis (PCR)
Molluscum contagiosum is a skin and mucous membrane, benign autoinoculable infection worldwide distribution caused by a poxvirus (Molluscipoxvirus) large, with genome dsDNA, which replicates in the cytoplasm of cells of the epidermis, it has not been grown in the laboratory and it has an experimental animal model, making it difficult to study its pathogenesis. Four genetic variants are currently differ. Of these genotypes, MCV1 is the most prevalent and causes infection in children, while MCV2 is more like sexually transmitted diseases and affects older patients.
The incidence is 2 to 10% of the population. Transmission occurs by contact interhuman person to person. In infected autoinoculation it may extend from scratching induced pruritus lesions. They have also been implicated fomites, and through towels, sponges, shaving utensils, and tattoo instruments.
We found some cases it may be possible to state that vertical transmission, manifesting infection among first days of life and 6 weeks after birth. In some cases described vertical transmission infection is manifested by the appearance of lesions on the scalp arranged in ring and associated with a prolonged primigravidas delivery that suggests the influence inoculation pressure scalp the cervix, which would expose cervical or vaginal secretions that may contain the virus.
Injury molluscum manifested as chronic injury proliferative, as a multiple nodules epidermal, small, painless, as papules small pearlescent, white or pink, smooth, bulged central umbilication, not affecting palms, or soles of the feet. These lesions persist for months or years, because the virus induces cellular immunity low, perhaps by some anti-inflammatory factors virulence. These lesions tend to resolve spontaneously over time. In the affects of AIDS is considered an opportunistic infection.
It has tried to make the possibility of transmission through swimming pool water, due to the discovery of some related sharing some pools outbreaks. Has studied the activities that may involve skin contact or water possibly contaminated with the virus, such as the use of swimming pools, baths, kindergartens, etc., These risk factors have been scientifically demonstrated not by not being able to grow the virus . Only we found statistically significant sharing bath sponges and bath towels with infected people relations. However, no relationship has been found between infection Molluscum contagiosum and swimming in private pools, public swimming pool, swimming beach or use private SPAs. Only found significant relationship with school swimming pool. Also with bath towels gyms, masseuses, fighters, or tattoo parlors.
Microbiological tests recommended
- Molecular detection by PCR.
Tests in IVAMI
- Molecular detection by PCR.
- Detection in water samples by concentration by filtration, followed by molecular detection by PCR.
- Lesion biopsy or swab impregnated by intense rubbing of lesions.
Preservation and sample shipping
- Refrigerated (preferred) for less than 2 days.
- Frozen: over 2 days.
- Molecular diagnosis: 24 to 48 hours.
- Detection in water pools, spas, ..: 48 to 72 hours.
Cost of the test
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