EN 14348: 2005. Quantitative suspension test for the evaluation of mycobactericidal activity of chemical disinfectants in the medical area Including instrument disinfectants. Test methods and requirements (phase 2, step 1).
Test accredited by ENAC (Spanish National Accreditation Entity), for standard mandatory microorganisms and additionals.
This test uses two test organisms required for mycobactericidal activity (Mycobacterium avium and Mycobacterium terrae) and one test organism for tuberculocidal activity (Mycobacterium terrae). The test should be performed at a mandatory temperature of 20ºC and for a mandatory time of 60 minutes. It can also be done with optional additional temperatures at intervals of 10ºC and for additional optional times of 5, 15 or 30 minutes. The test can be done under clean or dirty conditions. The product should be tested at least at three concentrrations including one in the non-active interval and another in the active interval. The product should be tested by the method of dilution-neutralization. To consider the mycobactericidal activity, the product must shows a log reduction of 4 in the number of viable counts.