EN 14204: 2013. Quantitative suspension test for the evaluation of mycobactericidal activity of chemical disinfectants and antiseptics used in the veterinary area. Test method and requirements (phase 2, step 1).

Test accredited by ENAC (National Accreditation Entitiy).
This test uses a forced organism (Mycobacterium avium). The test should be performed at a temperature of 10°C and mandatory for a mandatory 60 minutes. It can also be done with optional additional temperatures of 4, 20 or 40 and optional additional times for 1, 5, 10, 15, 30 or 120 minutes. The test can be performed under simulated conditions of low soil level or simulated conditions of high soil level. The product should be tested at least three levels including the non - active interval and another in the active interval. The product should be tested by the method of dilution-neutralization. To consider the mycobactericidal product must demonstrate a log reduction of 4 in the number of viable counts.