EN 1276: 2019 and BPR: 2023. Quantitative suspension test for the evaluation of bactericidal activity of chemical disinfectants and antiseptics used in food, industrial, domestic and institutional areas (phase 2, step 1) under specific conditions for PT5 of  BPR.

Test accredited by ENAC (Spanish National Accreditation Entity), for standard mandatory microorganisms and additionals, including for BPR requirements for PT1, PT2, PT4 and PT5.

This test is performed according to EN 1276 standard adapted according to the specific conditions of the BPR Guidance. The test uses four mandatory microorganisms Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus and Enterococcus hirae. The test must be performed at a mandatory temperature of 15ºC. The mandatory exposure duration and soiling conditions (quantity of Dissolved Organic Carbon or DOC) are stated in the table below, depending on the intended use of the product. For clean conditions 2 mg DOC/L must be used, while for dirty conditions 15 mg DOC/L need to be included.

Intended use of the product to evaluate

Contact time

Soiling conditions

PT5 Drinking water suppliers and their water distribution systems

Max 30 min

Clean for secondary disinfection /dirty for primary disinfection.

PT5 Raw water for individuals supply (1-2 premises)

Max 30 min


PT5 collective drinking water systems

25 min


PT5 water in reservoirs

As claimed

Clean for origin from drinking water supplier only/ dirty for origin raw water.

PT5 Water of undefined quality for small scale use (up to 5L/ person/day).

Max 30 min


PT5 Water for animals

Max 30 min (for continuous use the contact time is max 30 minutes. For reservoir water for animals the contact time should be as claimed).

Clean for origin from drinking water supplier only/ dirty for origin raw water.

The product must be assayed at least at three concentrations including one in the non-active range and other in the active range (these last two concentrations are selected by the laboratory performing the test, as the correspond to the positive and negative control, respectively). The test can be assayed by the dilution-neutralization method, or by the membrane filtration method. To consider the product as bactericidal a logarithmic reduction of 5 in the number of viable bacteria cell count, must be obtained.