Tests to determine MIC (Minimum Inhibitory Concentration), MBC (Minimum Bactericidal Concentration), MFC (Minimum Fungicidal Concentration) in disinfectant products.
Test not accredited in our laboratory.
The tests to determine the MIC, MBC and MFC for disinfectant products are carried out using a macrodilution procedure in test tubes, where the different concentrations of the product requested are prepared in a liquid culture medium and inoculated with the test microorganism(s). The tubes are incubated at a temperature that favours the growth of the requested test strain.
The method used to determine the MIC (Minimum Inhibitory Concentration) for a bacterial or fungal strain is the detection of turbidity. The presence of turbidity determines the growth of the inoculated microorganism. Through this process, the minimum concentration of the product that inhibits the growth of the microorganism in a given volume is determined.
The method used to determine the MBC (Minimum Bactericidal Concentration) or MFC (Minimum Fungicide Concentration) is carried out by neutralizing the previously indicated solution of the product and then seeding it in a solid medium appropriate for each microorganism being tested. In this way, the minimum amount of the product that induces the death of the microorganism in a given volume is determined.
The client must indicate the microorganisms against which they wish to test their product, as well as the concentrations at which they wish to test it. The maximum concentration of the product in the test is 50%, due to the dilution it undergoes in the test mixture.