Daphnia magna Acute Immobilization test (Daphnia magna Test Acute Toxicity, ISO 6341: 1996; OECD 202: 1984).
This test can be conducted in accordance to the specifications either of the following guidelines: ISO 6341: 1996; Method OECD 202: 1984; EPA-OPPTS-850-1010; RD 363/1995 of 10 March Annex V; EU guidelines 92/69 EEC (OJ L383 A).
In this test the acute toxicity to Daphnia magna can have soluble chemicals or chemicals that may remain in stable suspension or dispersion is determined. Likewise, it can be performed with or industrial waste effluents, treated or untreated after decantation, filtration or centrifugation, surface water or groundwater. The test determines the concentration at 24 hours immobilises 50% of the daphnia exposed EC50i-providing 24 h parameter, and when required immobilization at 48 hours (EC50i-48 h). The test is performed in two phases: in a first phase the concentration range is determined to test a stock solution of a substance, of an effluent, etc .; in the second phase, final assay concentrations immobilizing at least 3 percentage between 10 and 90% are calculated. All assays are always done in quadruplicate and requiring preliminary tests between the final, including positive and negative controls, about 400 copies of Daphnia magna. During the test the dissolved oxygen concentration and pH to be measured. It must be provided, if possible, the method for quantifying the chemical to be tested in the case of them.