Bordetella bronchiseptica - Culture; Molecular diagnosis (PCR)
Bordetella bronchiseptica is a Gram - negative bacteria in the form of highly contagious bacillus Bordetella, family Alcaligenaceae, with a worldwide distribution. Bordetella bronchiseptica primarily affects dogs, pigs, rabbits and laboratory animals, although it can infect cats and horses. In dogs , Bordetella bronchiseptica infection causes infectious canine tracheobronchitis (also known as kennel cough), a highly contagious respiratory disease. In pigs and rabbits, cause atrophic rhinitis and nasal disease, obstruction respectively. Bordetella bronchiseptica looks similar to its human counterpart, Bordetella pertussis behavior.
This microorganism, highly contagious, colonizes the host trachea. It is easily transmitted by direct contact or through the air, which can cause rapid spread of the disease. In addition, it may persist in the environment for long periods of time. After infection, Bordetella bronchiseptica joins tracheal ciliated epithelial cells, causing death later. Furthermore, Bordetella bronchiseptica is able to inhibit the function of leukocytes and induce apoptosis of alveolar macrophages, which is believed to contribute to the signs associated with the disease.
They have been reported infected with Bordetella bronchiseptica signs and symptoms including coughing, sneezing, runny nose, fever, anorexia, atrophic rhinitis, acute tracheobronchitis, conjunctivitis, mandibular lymphadenopathy, dyspnea, cyanosis and pneumonia animals. In mild cases, infected animals show clinical signs of recovery within 10 days after contracting the infection and disease without treatment can refer to 14 days after exposure.
It is rare that Bordetella bronchiseptica infects people, except for those immunocompromised individuals, such as people infected with HIV / AIDS, or people who have close contact with infected animals.
Recommended tests for diagnosis:
Conventional breeding can be performed, but has the disadvantage of slow development and identification. Antibodies are not interested because they take time to develop. Molecular methods (PCR), offer the advantage of providing a quick and specific diagnosis.
Tests in IVAMI:
- Molecular diagnosis (PCR), to detect DNA Bordetella bronchiseptica.
- Exudate nasopharyngeal swab taken.
Preservation and shipment of sample:
- Refrigerated (preferred) for less than 2.
- Frozen: over 2 days.
- Molecular diagnosis (PCR): 24 to 48 hours.
Cost of the test: