Repeated Dose Dermal Toxicity 21/28 days study. OECD 410: 1981.
Test with the Certificate of Good Laboratory Practices (GLPs).
This method consists of two tests: the main test and the limit test. This test can be performed on adult rats, rabbits or guinea pigs. At least 10 animals (5 females and 5 males) with healthy skin should be used, for each dose level (at least three).
The highest dose should cause toxic effects without causing death. The limit dose test corresponds to a dose of at least 1,000 mg/kg of body weight.
The method is based on the repeated application of the substance under study during a limiting period (several hours a day for 21/28 days). The test substance should be applied on at least 10 percent of the body surface.
The results for this study include the measurement and detailed daily observations of hematology, biochemistry and urinalysis, as well as macroscopic observations at necropsy and histopathology.