Proteolytic microorganisms (mesophilic and psychrophilic): Isolation in quantitative culture; Molecular identification (sequencing).
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Many proteolytic microorganisms may be involved, some mesophilic and others psychrophilic, belonging to various genera of bacteria, filamentous or yeast-like fungi. For example: Acinetobacter spp., Aeromonas spp., Alcaligenes spp., Bacillus spp., lactic acid bacteria (LAB, mainly of the genera Lactobacillus, Pediococcus, and Leuconostoc), Brevibacterium spp., Brochothrix thermosphacta, Clostridium spp., Flavobacterium spp., Micrococcus spp., Moraxella spp., Staphylococcus spp., Pseudomonas spp., Psychrobacter spp., some bacteria from the Enterobacteriaceae family, some filamentous fungi (Penicillium spp. and Mucor spp.), and some yeasts (e.g., Debaryomyces hansenii syn. Candida famata).
This involves growing in various culture media and incubating at a growth temperature for the development of mesophiles and at lower temperatures for the development of psychrophiles.